Yaman Yalçın, Aymaz Ramazan, Keleş Murat, Bay Veysel, Özüiçli Mehmet, Şenlik Bayram
Department of Biometry and Genetics, Sheep Breeding and Research Institute, Bandirma, Turkey.
Veterinary Faculty, Department of Parasitology, Uludag University, Bursa, Turkey.
Anim Biotechnol. 2021 Jun;32(3):375-380. doi: 10.1080/10495398.2020.1824921. Epub 2020 Sep 24.
Coccidiosis caused by spp. is a protozoan disease prevalent in farm animals, and it is responsible for serious economic losses especially in young animals. It has been popular to breed disease-resistant animals due to the concern about food safety, animal welfare, and public health. Toll-like receptor () gene family plays a key role in the innate immune system participating in host-antigen interaction, therefore, they are candidate genes for breeding disease-resistant animals. In the present study, possible genetic associations between gene coding variants and the presence of spp. in adult Turkish sheep were investigated. For this purpose, the presence of spp. in fecal samples from six native Turkish sheep were determined, and approximately 1450 bp region in the 3rd exon of the ovine gene was sequenced. Ten nonsynonymous and four synonymous single nucleotide polymorphisms (SNPs) were detected in the targeted region. Statistical analyses revealed that the SNP at the codon at 356 position encoding Leucine instead of Phenylalanine (F356L) was significantly associated with the presence of spp. It was found that the individuals carrying at least one Leucine amino acid sequence at this position have 2.3-fold more risk for the presence of spp.
由 spp. 引起的球虫病是一种在农场动物中普遍存在的原生动物疾病，尤其在幼龄动物中会造成严重的经济损失。由于对食品安全、动物福利和公共卫生的关注，培育抗病动物已成为一种趋势。Toll样受体（）基因家族在参与宿主 - 抗原相互作用的先天免疫系统中起关键作用，因此，它们是培育抗病动物的候选基因。在本研究中，调查了成年土耳其绵羊中 基因编码变异与 spp. 存在之间可能的遗传关联。为此，测定了六只土耳其本土绵羊粪便样本中 spp. 的存在情况，并对绵羊 基因第3外显子中约1450 bp的区域进行了测序。在目标区域检测到10个非同义单核苷酸多态性（SNP）和4个同义SNP。统计分析表明，编码亮氨酸而非苯丙氨酸（F356L）的第356位密码子处的SNP与 spp. 的存在显著相关。发现在此位置携带至少一个亮氨酸氨基酸序列的个体感染 spp. 的风险高出2.3倍。
