Petrenko V A, Kipriianov S M, Semenova L N, Boldyrev A N
Bioorg Khim. 1987 Mar;13(3):344-9.
The possibility to use the E. coli intact DNA polymerase I in the oligonucleotide-directed site-specific mutagenesis of DNA has been studied. Optimal conditions of the extension activity of this enzyme were found. We have shown that the substitution of the Klenow fragment of the E. coli DNA polymerase by the intact DNA polymerase I did not decrease the efficiency and fidelity of the oligonucleotide-directed mutagenesis.
研究了在DNA的寡核苷酸定向位点特异性诱变中使用大肠杆菌完整DNA聚合酶I的可能性。发现了该酶延伸活性的最佳条件。我们已经表明，用完整的DNA聚合酶I替代大肠杆菌DNA聚合酶的Klenow片段不会降低寡核苷酸定向诱变的效率和保真度。
