Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
FEMS Microbiol Ecol. 2020 Nov 26;96(12). doi: 10.1093/femsec/fiaa194.
Symbiotic microorganisms in invertebrates play vital roles in host ecology and evolution. Cardinium, a common intracellular symbiont, is transinfected into the important agricultural pest Nilaparvata lugens (rice brown planthopper) to regulate its reproduction, but how this impacts its microbial community is unknown. Here, we characterized the bacterial microbiota from N. lugens, with or without Cardinium, at different developmental stages and in various adult tissues using 16S ribosomal ribonucleic acid (16S rRNA) gene sequencing. Upon infection with Cardinium, we found that microbial diversity in the different developmental stages of N. lugens (especially females), and in female midguts and male testes, was lower than that in the uninfected control. There was a negative correlation between Cardinium and most related genera and between Bacteroidetes and Proteobacteria. Although the microbial structure varied during Cardinium infection, Acinetobacter spp. were a core microbiome genus. The Cardinium infection enhanced the relative density of midgut-associated Acinetobacter spp., with both bacteria exhibiting tissue-specific tropism. In addition, this infection caused the changes of main microbial functions in N. lugens. These results offer insights into the effects of alien (i.e. newly introduced from other organism) Cardinium infection on N. lugens-associated microbiotas, aiding in the development of transinfected endosymbionts for pest control.
无脊椎动物体内的共生微生物在宿主生态和进化中起着至关重要的作用。卡多体（Cardinium）是一种常见的细胞内共生体，它被转染到重要的农业害虫褐飞虱（Nilaparvata lugens）中，以调节其繁殖，但这如何影响其微生物群落尚不清楚。在这里，我们使用 16S 核糖体核糖核酸（16S rRNA）基因测序技术，对携带或不携带卡多体的不同发育阶段和不同成虫组织的褐飞虱的细菌微生物群进行了特征描述。在感染卡多体后，我们发现褐飞虱不同发育阶段（尤其是雌性）以及雌性中肠和雄性睾丸中的微生物多样性低于未感染对照。卡多体与大多数相关属之间以及与变形菌门和厚壁菌门之间呈负相关。尽管在卡多体感染过程中微生物结构发生了变化，但不动杆菌属（Acinetobacter）是核心微生物组属。卡多体感染增强了与中肠相关的不动杆菌属的相对密度，这两种细菌都具有组织特异性嗜性。此外，这种感染导致褐飞虱主要微生物功能的变化。这些结果深入了解了外来（即从其他生物体新引入的）卡多体感染对褐飞虱相关微生物群的影响，有助于开发用于害虫防治的转染内共生体。
