School of Fundamental Science and Technology, Graduate School of Science and Technology, Keio University, Hiyoshi, Yokohama 223-8522, Japan.
Department of Biology, School of Medicine, Keio University, Hiyoshi, Yokohama 223-8521, Japan,
Zoolog Sci. 2020 Oct;37(5):476-495. doi: 10.2108/zs190154.
Presently, more than 40 species of the genus Doyère, 1840 (Tardigrada: Eutardigrada: Apochela: Milnesiidae) have been described. In Japan, however, almost all records of milnesiid tardigrades should be re-examined with the current criteria on the taxonomy of this genus, except for one species, the recently described Morek, Suzuki, Schill, Georgiev, Yankova, Marley, and Michalczyk, 2019. In this study, we found two species, sp. nov. and Doyère, 1840, from three southern islands and two cold regions in Japan, respectively. sp. nov., having dorsal sculpturing, exhibits an early positive change in claw configuration. On the other hand, s.s. from Japan has an early negative claw configuration change, as has been reported in a recent study on the neotype population of this species. We performed DNA barcoding for both species, which indicated that sp. nov. has a close affinity with an undescribed species collected from Brazil, and that from Japan represents the recently described subclade that contains specimens from Poland, Hungary, and Russia. The chromosome numbers were 2n = 14 in sp. nov. and 2n = 10 in . We detected at least three species of the genus present in Japan. Our results advance the investigation of the relationship between phylogenetic position and characteristic morphology as well as expand the known geographic range of .
目前，已经描述了 40 多种 Doyère 属（缓步动物门：Eutardigrada：Apochela：Milnesiidae）物种。然而，在日本，除了一种最近描述的 Morek、Suzuki、Schill、Georgiev、Yankova、Marley 和 Michalczyk, 2019 外，几乎所有 Milnesiidae 缓步动物的记录都应该用当前的分类标准重新检查。在这项研究中，我们从日本的三个南部岛屿和两个寒冷地区分别发现了两个物种， sp. nov. 和 Doyère, 1840。sp. nov. 具有背侧纹饰，表现出早期正爪构型的变化。另一方面，来自日本的 s.s. 具有早期的负爪构型变化，正如最近对该物种的模式种群的研究报告的那样。我们对这两个物种进行了 DNA 条码分析，结果表明 sp. nov. 与从巴西采集的未描述物种密切相关，而来自日本的则代表了最近描述的包含来自波兰、匈牙利和俄罗斯标本的亚分支。sp. nov. 的染色体数为 2n = 14， 为 2n = 10。我们在日本检测到至少三种 Doyère 属的物种。我们的结果推进了对系统发育位置和特征形态之间关系的研究，并扩大了该属的已知地理分布范围。
