Department of Orthodontics, University Hospital Jena, Leutragraben 3, 07743, Jena, Germany.
Department of Orthodontics, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany.
Sci Rep. 2020 Sep 24;10(1):15706. doi: 10.1038/s41598-020-72736-7.
Alveolar bone (AB) remodeling is necessary for the adaption to mechanical stimuli occurring during mastication and orthodontic tooth movement (OTM). Thereby, bone degradation and assembly are strongly regulated processes that can be altered in obese patients. Further, increased fatty acids (FA) serum levels affect bone remodeling cells and we, therefore, investigated whether they also influence the function of periodontal ligament fibroblast (PdLF). PdLF are a major cell type regulating the differentiation and function of osteoblasts and osteoclasts localized in the AB. We stimulated human PdLF (HPdLF) in vitro with palmitic (PA) or oleic acid (OA) and analyzed their metabolic activity, growth, survival and expression of osteogenic markers and calcium deposits. Our results emphasize that PA increased cell death of HPdLF, whereas OA induced their osteoblastic differentiation. Moreover, quantitative expression analysis of OPG and RANKL revealed altered levels in mechanically stimulated PA-treated HPdLF. Furthermore, osteoclasts stimulated with culture medium of mechanical stressed FA-treated HPdLF revealed significant changes in cell differentiation upon FA-treatment. For the first time, our results highlight a potential role of specific FA in the function of HPdLF-modulated AB remodeling and help to elucidate the complex interplay of bone metabolism, mechanical stimulation and obesity-induced alterations.
牙槽骨（AB）重塑是咀嚼和正畸牙齿移动（OTM）过程中适应机械刺激所必需的。因此，骨降解和组装是受强烈调节的过程，在肥胖患者中可以改变。此外，脂肪酸（FA）血清水平升高会影响骨重塑细胞，因此我们研究了它们是否也会影响牙周膜成纤维细胞（PdLF）的功能。PdLF 是一种主要的细胞类型，可调节位于 AB 中的成骨细胞和破骨细胞的分化和功能。我们在体外用人 PdLF（HPdLF）刺激棕榈酸（PA）或油酸（OA），并分析其代谢活性、生长、存活以及成骨标记物和钙沉积的表达。我们的结果强调，PA 增加了 HPdLF 的细胞死亡，而 OA 则诱导其成骨细胞分化。此外，对 OPG 和 RANKL 的定量表达分析表明，机械刺激 PA 处理的 HPdLF 中存在改变的水平。此外，用机械刺激 FA 处理的 HPdLF 的培养物刺激破骨细胞，在 FA 处理时显示出细胞分化的显著变化。我们的研究结果首次强调了特定 FA 在 HPdLF 调节的 AB 重塑功能中的潜在作用，并有助于阐明骨代谢、机械刺激和肥胖诱导的改变之间的复杂相互作用。
