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一种用于探索颗粒诱导的肺损伤挥发生物标志物的新型肺泡细胞模型。

A novel lung alveolar cell model for exploring volatile biomarkers of particle-induced lung injury.

机构信息

School of Respiratory Therapy, Taipei Medical University, Taipei, Taiwan.

Institute of Environmental and Occupational Health Sciences, National Taiwan University College of Public Health, Taipei, Taiwan.

出版信息

Sci Rep. 2020 Sep 24;10(1):15700. doi: 10.1038/s41598-020-72825-7.

DOI:10.1038/s41598-020-72825-7
PMID:32973288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7515894/
Abstract

Quartz can increase oxidative stress, lipid peroxidation, and inflammation. The objective of this study was to explore the volatile biomarkers of quartz-induced lung injury using a lung alveolar cell model. We exposed the human alveolar A549 cell line to 0, 200, and 500 μg/mL quartz particles for 24 h and used gas chromatography-mass spectrometry to measure the volatile metabolites in the headspace air of cells. We identified ten volatile metabolites that had concentration-response relationships with particles exposure, including 1,2,4-oxadiazole, 5-(4-nitrophenyl)-3-phenyl- (CAS: 28825-12-9), 2,6-dimethyl-6-trifluoroacetoxyoctane (CAS: 61986-67-2), 3-buten-1-amine, N,N-dimethyl- (CAS: 55831-89-5), 2-propanol, 2-methyl- (CAS: 75-65-0), glycolaldehyde dimethyl acetal (CAS: 30934-97-5), propanoic acid, 2-oxo-, ethyl ester (CAS: 617-35-6), octane (CAS: 111-65-9), octane, 3,3-dimethyl- (CAS: 4110-44-5), heptane, 2,3-dimethyl- (CAS: 3074-71-3) and ethanedioic acid, bis(trimethylsilyl) ester (CAS: 18294-04-7). The volatile biomarkers are generated through the pathways of propanoate and nitrogen metabolism. The volatile biomarkers of the alkanes and methylated alkanes are related to oxidative and lipid peroxidation of the cell membrane. The lung alveolar cell model has the potential to explore the volatile biomarkers of particulate-induced lung injury.

摘要

石英可增加氧化应激、脂质过氧化和炎症。本研究旨在通过肺泡细胞模型探索石英诱导的肺损伤的挥发性生物标志物。我们将人肺泡 A549 细胞系暴露于 0、200 和 500μg/ml 的石英颗粒中 24 小时，并用气相色谱-质谱联用仪测量细胞内空气的挥发性代谢物。我们鉴定出十种与颗粒暴露呈浓度反应关系的挥发性代谢物，包括 1,2,4-恶二唑、5-(4-硝基苯基)-3-苯基-(CAS:28825-12-9)、2,6-二甲基-6-三氟乙酰氧基辛烷(CAS:61986-67-2)、3-丁烯-1-胺，N,N-二甲基-(CAS:55831-89-5)、2-丙醇，2-甲基-(CAS:75-65-0)、乙二醇二甲缩醛(CAS:30934-97-5)、丙酸，2-氧代-，乙酯(CAS:617-35-6)、辛烷(CAS:111-65-9)、辛烷，3,3-二甲基-(CAS:4110-44-5)、庚烷，2,3-二甲基-(CAS:3074-71-3)和乙二酸，双(三甲基硅基)酯(CAS:18294-04-7)。这些挥发性生物标志物是通过丙酸和氮代谢途径生成的。烷烃和甲基化烷烃的挥发性生物标志物与细胞膜的氧化和脂质过氧化有关。肺泡细胞模型有潜力探索颗粒诱导的肺损伤的挥发性生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/61b42f05e6b2/41598_2020_72825_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/c059db5101f2/41598_2020_72825_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/e0ec5cf2e90a/41598_2020_72825_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/0bb0d927c570/41598_2020_72825_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/61b42f05e6b2/41598_2020_72825_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/c059db5101f2/41598_2020_72825_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/e0ec5cf2e90a/41598_2020_72825_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/0bb0d927c570/41598_2020_72825_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/169b/7515894/61b42f05e6b2/41598_2020_72825_Fig4_HTML.jpg

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