茄碱减弱乙酰胆碱诱导的肝癌细胞迁移和侵袭。

Solanine Attenuates Hepatocarcinoma Migration and Invasion Induced by Acetylcholine.

机构信息

Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.

出版信息

Integr Cancer Ther. 2020 Jan-Dec;19:1534735420909895. doi: 10.1177/1534735420909895.

AIM

Evidence has provided an explanation of the correlation between the nervous system and the tumor microenvironment. Neurotransmitters may be involved in different aspects of cancer progression. The glycoalkaloid solanine has been reported to suppress neural signaling pathways and exists in numerous plants, including , which have been demonstrated to inhibit cancer cell proliferation.

METHODS

We evaluated the potentials of solanine on inhibiting acetylcholine-induced cell proliferation and migration in hepatocellular carcinoma cells.

RESULTS

The results indicated that solanine markedly attenuated cell proliferation and migration via inhibiting epithelial-mesenchymal transition and matrix metalloproteinases in acetylcholine-treated Hep G2 cells. In addition, exosomes derived from acetylcholine-treated Hep G2 cells were isolated, and solanine showed inhibiting effects of extrahepatic metastasis on blocking cell proliferation in exosome-treated A549 lung carcinoma cells through regulating microRNA-21 expression.

CONCLUSION

Solanine has strong potential for application in integrative cancer therapy.

目的

有证据表明神经系统与肿瘤微环境之间存在关联。神经递质可能参与癌症进展的不同方面。已报道糖苷生物碱龙葵碱可抑制神经信号通路，并且存在于许多植物中，包括，其已被证明可抑制癌细胞增殖。

方法

我们评估了龙葵碱抑制乙酰胆碱诱导的肝癌细胞增殖和迁移的潜力。

结果

结果表明，龙葵碱通过抑制乙酰胆碱处理的 Hep G2 细胞中的上皮-间充质转化和基质金属蛋白酶，显著减弱细胞增殖和迁移。此外，分离出由乙酰胆碱处理的 Hep G2 细胞衍生的外泌体，龙葵碱通过调节 microRNA-21 的表达，显示出对细胞增殖的抑制作用，从而阻断外泌体处理的 A549 肺癌细胞的肝外转移。