Martikainen P, Härkönen P, Vanhala T, Mäkelä S, Viljanen M, Suominen J
Endocrinology. 1987 Aug;121(2):604-11. doi: 10.1210/endo-121-2-604.
The synthesis and accumulation of prostatein, a major secretory protein of the rat ventral prostate, was examined in organ culture conditions. For the quantitation of this protein in the medium, a sensitive enzyme immunoassay was developed. The rat ventral prostate could be maintained in organ culture in defined medium for at least 2 weeks. Morphologically the changes in explants cultured without hormones resembled those of castration. These involutive changes could be postponed by testosterone and totally prevented by a combination of testosterone, corticosterone, and insulin in the culture medium. Newly synthesized prostatein, studied by fluorography of [35S] methionine-labeled proteins, accumulated only in the presence of testosterone. Its synthesis also took place in cultured prostate derived from castrated rats. Neither corticosterone nor insulin alone could sustain prostatein synthesis. Insulin increased the testosterone-dependent prostatein synthesis in the beginning of culture, but later, inhibition, rather than stimulation, could be noted. Corticosterone increased the testosterone-dependent synthesis of prostatein throughout the culture. The results show that organ culture of adult rat ventral prostate provides an in vitro model for studies of differentiated prostatic function.
在器官培养条件下，对大鼠腹侧前列腺的主要分泌蛋白前列腺蛋白的合成与积累进行了研究。为了定量培养基中的这种蛋白质，开发了一种灵敏的酶免疫测定法。大鼠腹侧前列腺可以在限定培养基中进行器官培养至少2周。在形态学上，无激素培养的外植体的变化类似于去势后的变化。这些退化性变化可被睾酮推迟，并且在培养基中加入睾酮、皮质酮和胰岛素的组合可完全防止。通过对[35S]甲硫氨酸标记的蛋白质进行放射自显影研究发现，新合成的前列腺蛋白仅在有睾酮存在时积累。其合成也发生在去势大鼠的培养前列腺中。单独的皮质酮或胰岛素都不能维持前列腺蛋白的合成。胰岛素在培养开始时增加了睾酮依赖性前列腺蛋白的合成，但后来，观察到的是抑制而非刺激作用。在整个培养过程中，皮质酮增加了睾酮依赖性前列腺蛋白的合成。结果表明，成年大鼠腹侧前列腺的器官培养为研究分化的前列腺功能提供了一个体外模型。
