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M13前体蛋白组装到微粒体中需要ATP的步骤与前体蛋白转运能力的维持有关。

The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

作者信息

Wiech H, Sagstetter M, Müller G, Zimmermann R

出版信息

EMBO J. 1987 Apr;6(4):1011-6. doi: 10.1002/j.1460-2075.1987.tb04853.x.

DOI:10.1002/j.1460-2075.1987.tb04853.x
PMID:3297670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553497/
Abstract

M13 procoat protein is processed to transmembrane coat protein by dog pancreas microsomes after completion of synthesis and in the absence of the signal recognition particle (SRP)/docking protein system. ATP is required for fast and efficient processing of procoat protein by microsomes in a reticulocyte lysate. Requirement for ATP is also observed in the absence of ribosomes or docking protein. This indicates the existence of a unique assembly pathway for procoat protein into microsomes which depends on ATP but does not depend on the SRP/docking protein and ribosome/ribosome receptor systems. We suggest that the ATP requirement is linked to a so far unknown component of the reticulocyte lysate, acting on transport competence of precursor proteins.

摘要

M13前体蛋白在合成完成后且在没有信号识别颗粒（SRP）/对接蛋白系统的情况下，被犬胰腺微粒体加工成跨膜外壳蛋白。ATP是网织红细胞裂解液中微粒体快速高效加工前体蛋白所必需的。在没有核糖体或对接蛋白的情况下也观察到对ATP的需求。这表明前体蛋白进入微粒体存在一条独特的组装途径，该途径依赖于ATP，但不依赖于SRP/对接蛋白和核糖体/核糖体受体系统。我们认为对ATP的需求与网织红细胞裂解液中一个迄今未知的成分有关，该成分作用于前体蛋白的转运能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/e829563387d7/emboj00244-0180-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/9b5ef57507ff/emboj00244-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/ffc1251626f0/emboj00244-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/54f5d77988c6/emboj00244-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/5f9e79d5e229/emboj00244-0180-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/e829563387d7/emboj00244-0180-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/9b5ef57507ff/emboj00244-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/ffc1251626f0/emboj00244-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/54f5d77988c6/emboj00244-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/5f9e79d5e229/emboj00244-0180-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e654/553497/e829563387d7/emboj00244-0180-c.jpg

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The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.M13前体蛋白组装到微粒体中需要ATP的步骤与前体蛋白转运能力的维持有关。
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本文引用的文献

1
Secretion in yeast: translocation and glycosylation of prepro-alpha-factor in vitro can occur via an ATP-dependent post-translational mechanism.酵母中的分泌：体外前原α因子的转运和糖基化可通过一种依赖ATP的翻译后机制发生。
EMBO J. 1986 May;5(5):1031-6. doi: 10.1002/j.1460-2075.1986.tb04318.x.
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Membrane assembly: posttranslational insertion of M13 procoat protein into E. coli membranes and its proteolytic conversion to coat protein in vitro.膜组装：M13原衣壳蛋白在翻译后插入大肠杆菌膜中并在体外被蛋白水解转化为衣壳蛋白。
Cell. 1981 May;24(2):437-41. doi: 10.1016/0092-8674(81)90334-2.
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Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.
J Cell Sci. 2012 Aug 1;125(Pt 15):3612-20. doi: 10.1242/jcs.102608. Epub 2012 Apr 14.
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A microsomal ATP-binding protein involved in efficient protein transport into the mammalian endoplasmic reticulum.一种参与将蛋白质高效转运至哺乳动物内质网的微粒体ATP结合蛋白。
EMBO J. 1996 Dec 16;15(24):6931-42.
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Polypeptide translocation machinery of the yeast endoplasmic reticulum.酵母内质网的多肽转运机制
Experientia. 1996 Dec 15;52(12):1042-9. doi: 10.1007/BF01952100.
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Import of honeybee prepromelittin into the endoplasmic reticulum: energy requirements for membrane insertion.
EMBO J. 1988 Mar;7(3):639-48. doi: 10.1002/j.1460-2075.1988.tb02858.x.
7
Seventy-kilodalton heat shock proteins and an additional component from reticulocyte lysate stimulate import of M13 procoat protein into microsomes.70千道尔顿热休克蛋白和来自网织红细胞裂解物的另一种成分刺激M13原衣壳蛋白导入微粒体。
EMBO J. 1988 Sep;7(9):2875-80. doi: 10.1002/j.1460-2075.1988.tb03144.x.
8
ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.原OmpA能自发折叠成一种膜组装活性状态，触发因子可使其稳定。
EMBO J. 1988 Jun;7(6):1831-5. doi: 10.1002/j.1460-2075.1988.tb03015.x.
9
DCCD inhibits protein translocation into plasma membrane vesicles from Escherichia coli at two different steps.二环己基碳二亚胺（DCCD）在两个不同步骤抑制蛋白质转运到来自大肠杆菌的质膜囊泡中。
EMBO J. 1987 Dec 1;6(12):3855-61. doi: 10.1002/j.1460-2075.1987.tb02723.x.
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4
Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.膜组装机制：能量毒物对可溶性M13噬菌体原衣壳转化为膜结合衣壳蛋白的影响。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):827-31. doi: 10.1073/pnas.77.2.827.
5
M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms.M13前衣壳蛋白和前免疫球蛋白具有共同的加工特异性，但使用不同的膜受体机制。
Proc Natl Acad Sci U S A. 1983 May;80(10):2809-13. doi: 10.1073/pnas.80.10.2809.
6
Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bacterial leader peptidase.M13前衣壳蛋白快速不对称组装到含有细菌前导肽酶的脂质体中。
J Biol Chem. 1983 Feb 10;258(3):1895-900.
7
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.功能性信使核糖核酸通过克隆的互补脱氧核糖核酸的SP6体外转录产生。
Nucleic Acids Res. 1984 Sep 25;12(18):7057-70. doi: 10.1093/nar/12.18.7057.
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Pushing the signal hypothesis: what are the limits?
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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
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Protein translocation across the endoplasmic reticulum.蛋白质在内质网上的转运
Cell. 1984 Aug;38(1):5-8. doi: 10.1016/0092-8674(84)90520-8.