Wiech H, Sagstetter M, Müller G, Zimmermann R
EMBO J. 1987 Apr;6(4):1011-6. doi: 10.1002/j.1460-2075.1987.tb04853.x.
M13 procoat protein is processed to transmembrane coat protein by dog pancreas microsomes after completion of synthesis and in the absence of the signal recognition particle (SRP)/docking protein system. ATP is required for fast and efficient processing of procoat protein by microsomes in a reticulocyte lysate. Requirement for ATP is also observed in the absence of ribosomes or docking protein. This indicates the existence of a unique assembly pathway for procoat protein into microsomes which depends on ATP but does not depend on the SRP/docking protein and ribosome/ribosome receptor systems. We suggest that the ATP requirement is linked to a so far unknown component of the reticulocyte lysate, acting on transport competence of precursor proteins.
M13前体蛋白在合成完成后且在没有信号识别颗粒(SRP)/对接蛋白系统的情况下,被犬胰腺微粒体加工成跨膜外壳蛋白。ATP是网织红细胞裂解液中微粒体快速高效加工前体蛋白所必需的。在没有核糖体或对接蛋白的情况下也观察到对ATP的需求。这表明前体蛋白进入微粒体存在一条独特的组装途径,该途径依赖于ATP,但不依赖于SRP/对接蛋白和核糖体/核糖体受体系统。我们认为对ATP的需求与网织红细胞裂解液中一个迄今未知的成分有关,该成分作用于前体蛋白的转运能力。