Watts C, Wickner W, Zimmermann R
Proc Natl Acad Sci U S A. 1983 May;80(10):2809-13. doi: 10.1073/pnas.80.10.2809.
Bacteriophage M13 procoat is accurately processed to transmembrane coat protein by salt-washed or N-ethylmaleimide-treated rough microsomes from dog pancreas. These treatments inhibit the processing of eukaryotic secreted protein precursors. M13 procoat can assemble into dog pancreas microsomes post-translationally. Thus, the microsomal proteins needed for assembly may be determined by the nature of the precursor protein itself. These results, and our finding that the mouse IgG kappa chain fragment precursor is processed by Escherichia coli leader peptidase, also suggest that the cleavage specificity of leader (signal) peptidases and the properties of preproteins that render them suitable for cleavage have been conserved during evolution.
噬菌体M13前衣壳蛋白能被来自狗胰腺的经盐洗涤或N-乙基马来酰亚胺处理的糙面微粒体准确加工成跨膜衣壳蛋白。这些处理会抑制真核分泌蛋白前体的加工。M13前衣壳蛋白能在翻译后组装到狗胰腺微粒体中。因此，组装所需的微粒体蛋白可能由前体蛋白本身的性质决定。这些结果，以及我们发现小鼠IgG κ链片段前体可被大肠杆菌前导肽酶加工，也表明前导（信号）肽酶的切割特异性以及使前体蛋白适合切割的特性在进化过程中得到了保留。
