College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong, 271018, PR China.
College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, 61 Daizong Street, Tai'an, Shandong, 271018, PR China.
Plant Physiol Biochem. 2020 Nov;156:233-241. doi: 10.1016/j.plaphy.2020.09.009. Epub 2020 Sep 12.
Hydrogen sulfide (HS) plays an important role in the plant salt stress response. The main component of salt stress is neutral salt (NaCl); NaHCO and NaCO play a key role in soil alkaline due to the influence of pH. Malus hupehensis Rehd. var. pingyiensis Jiang (Pingyi Tiancha, PYTC) is a salt-sensitive apple rootstock. Seedlings of PYTC pretreated with NaHS (an HS donor) were exposed to an alkaline salt solution, and then the plant growth, root architecture, oxidative damage, Na/K homeostasis and gene expression of MhSOS1 and MhSKOR were investigated. The results showed that NaHS pretreatment increased the endogenous HS content in seedlings, significantly alleviated the alkaline salt stress-induced growth inhibition and oxidative damage by inducing antioxidant enzymes activities, and sustained the root activity and root architecture of PYTC in the alkaline salt solution. NaHS pretreatment significantly decreased the root Na content and increased K content to maintain the homeostasis of Na/K, and effect the expression of MhSOS1 and MhSKOR at the transcription level in the presence of the alkaline salt. Our study reveals that application of HS could mitigate the toxic effect of alkaline salt stress on Malus hupehensis seedlings, thus providing a foundation for improved plant tolerance to alkaline salt stress.
硫化氢 (HS) 在植物盐胁迫响应中发挥重要作用。盐胁迫的主要成分是中性盐 (NaCl)；由于 pH 的影响，NaHCO 和 NaCO 在土壤碱性中起关键作用。平邑甜茶 (Malus hupehensis Rehd. var. pingyiensis Jiang, PYTC) 是一种对盐敏感的苹果砧木。用 HS 供体 NaHS 预处理的 PYTC 幼苗暴露于碱性盐溶液中，然后研究植物生长、根系结构、氧化损伤、Na/K 稳态和 MhSOS1 和 MhSKOR 的基因表达。结果表明，NaHS 预处理增加了幼苗中的内源性 HS 含量，通过诱导抗氧化酶活性，显著缓解碱性盐胁迫引起的生长抑制和氧化损伤，并维持 PYTC 在碱性盐溶液中的根系活力和根系结构。NaHS 预处理显著降低了根中的 Na 含量，增加了 K 含量，以维持 Na/K 的稳态，并在碱性盐存在的情况下影响 MhSOS1 和 MhSKOR 的转录水平表达。我们的研究表明，HS 的应用可以减轻碱性盐胁迫对平邑甜茶幼苗的毒害作用，从而为提高植物对碱性盐胁迫的耐受性提供了基础。
