Wei Guo Qin, Cao Hui, Sun Yu Gang, Deng Bo, Zhang Wei Wei, Yang Hong Qiang
College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
Shandong Institute of Pomology, Tai'an 271000, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2017 Oct;28(10):3267-3273. doi: 10.13287/j.1001-9332.201710.006.
The root architecture, leaf reactive oxygen species and photosynthetic characteristics of potted Malus hupehensis seedlings were investigated after 10 days of waterlogging under five concentrations of hydrogen sulfide (H2S) donor sodium hydrosulfide (NaSH, 0, 0.02, 0.05, 0.1, 0.2 mmol·L) and NaSH with scavenger hypotaurine (HT). The results showed that the root length, root surface area, root volume, root diameter, root top number, fractal dimension, first lateral roots and root activity all increased, the leaf superoxide anion (O2) accumulation and hydrogen pero-xide (H2O2) content both decreased, the leaf P, T, g, WUE and CUE all raised, the plant height and biomass both increased when the 0.02-0.1 mmol·L NaHS were added to the waterlogging environment. Adding 0.1 mmol·L NaHS showed the best effects. All these parameters dropped back to the level of waterlogging alone when NaHS concentration was 0.2 mmol·L or HT was added to the 0.1 mmol·L NaHS solution. These suggested that applying an appropriate amount of H2S could effectively alleviate the inhibition of soil waterlogging on the root growth of M. hupehensis and relieve the effects of waterlogging stress on photosynthesis and plant growth by reducing the production of reactive oxygen species and increasing CUE.
在五种浓度的硫化氢（H₂S）供体硫氢化钠（NaSH，0、0.02、0.05、0.1、0.2 mmol·L⁻¹）以及添加清除剂次牛磺酸（HT）的NaSH处理下，对盆栽湖北海棠幼苗进行10天的淹水试验后，研究了其根系结构、叶片活性氧和光合特性。结果表明，当在淹水环境中添加0.02 - 0.1 mmol·L⁻¹的NaHS时，根长、根表面积、根体积、根直径、根尖数、分形维数、一级侧根和根系活力均增加，叶片超氧阴离子（O₂⁻）积累量和过氧化氢（H₂O₂）含量均降低，叶片净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）、水分利用效率（WUE）和碳利用效率（CUE）均提高，株高和生物量均增加。添加0.1 mmol·L⁻¹的NaHS效果最佳。当NaHS浓度为0.2 mmol·L⁻¹或在0.1 mmol·L⁻¹的NaHS溶液中添加HT时，所有这些参数均降至单独淹水水平。这些结果表明，施加适量的H₂S可以有效缓解土壤淹水对湖北海棠根系生长的抑制，并通过减少活性氧的产生和提高CUE来减轻淹水胁迫对光合作用和植物生长的影响。
