鼠源和人源骨细胞的分离。

Isolation of Murine and Human Osteocytes.

机构信息

Indiana Center for Musculoskeletal Health and Department of Anatomy, Cell Biology and Physiology, Indiana University, Indianapolis, IN, USA.

Engineering Systems Inc., Charlotte, NC, USA.

出版信息

Methods Mol Biol. 2021;2221:3-13. doi: 10.1007/978-1-0716-0989-7_1.

DOI:10.1007/978-1-0716-0989-7_1

PMID:32979194

Abstract

Osteocytes are thought to be the mechanosensors of bone by sensing mechanical loads imposed upon the bone and transmitting these signals to the other bone cells to initiate bone modeling and remodeling. The location of osteocytes deep within bone is ideal for their function. However, this location makes the study of osteocytes in vivo technically difficult. There are several methods for obtaining and culturing primary osteocytes for in vitro experiments and ex vivo observation. In this chapter, several proven methods are discussed including the isolation of avian osteocytes from chicks and osteocytes from calvaria and long bones of young mice. A detailed protocol for the isolation of osteocytes from hypermineralized bone of mature and aged animals is provided. In addition, a modified version of this protocol that can be used to isolate osteocytes from human trabecular bone is described.

摘要

骨细胞被认为是骨骼的机械感受器，通过感知施加在骨骼上的机械负荷，并将这些信号传递给其他骨骼细胞，从而启动骨建模和重塑。骨细胞位于骨骼深处的位置非常适合其功能。然而，这种位置使得在体内研究骨细胞在技术上具有挑战性。有几种方法可用于获得和培养原代骨细胞进行体外实验和离体观察。在本章中，讨论了几种经过验证的方法，包括从小鸡中分离出禽骨细胞，以及从小鼠的颅骨和长骨中分离出骨细胞。提供了从成熟和老年动物的高矿化骨中分离骨细胞的详细方案。此外，还描述了该方案的改良版本，可用于从人小梁骨中分离骨细胞。