从椎间盘分离核髓和纤维环细胞。

Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc.

机构信息

Laboratory for Experimental Orthopedics, Department of Orthopedic Surgery, Maastricht University, Maastricht, The Netherlands.

Department of Molecular Genetics, Maastricht University, Maastricht, The Netherlands.

出版信息

Methods Mol Biol. 2021;2221:41-52. doi: 10.1007/978-1-0716-0989-7_4.

DOI:10.1007/978-1-0716-0989-7_4

PMID:32979197

Abstract

Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.

摘要

从椎间盘分离出的细胞常用于体外实验。在使用手术剩余材料或老年供体标本时，正确分离纤维环和髓核的椎间盘组织特别具有挑战性。此外，仅有少数报道使用谱系对照来验证组织来源。本文介绍了一种从人源和牛源分离椎间盘细胞的方法。