Fisheries College, Guangdong Ocean University, Zhanjiang, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, CAFS, Qingdao, 266071, China; Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, CAFS, Qingdao, 266071, China; Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China.
Gene Expr Patterns. 2020 Dec;38:119148. doi: 10.1016/j.gep.2020.119148. Epub 2020 Sep 24.
Inhibin plays important roles in vertebrate reproduction and development. In this study, we have cloned two genes encoding inhibin subunits, inhα and ihnβb, in Chinese tongue sole. inhα consists of 1032 bp, encoding a 343 amino-acid protein. inhβb is composed of 1275 bp, encoding a 424 amino-acid protein. Phylogenetic tree analysis indicated that INHα and INHβB were independently evolved. qPCR showed that inhα expression of in male testis was higher than that in ovary and pseudomale testis, while the expression of inhβb in ovary was higher than that in male and pseudomale testis. During gonadal developmental stages, inhα expression reached highest at 120 days post hatching (dph) both in ovary and testis, then showed decline in ovary but it was first decreased and then increased in the testis. Similarly, inhβb expression in ovary was low at 50-80 dph. At 120 dph, its expression was significantly increased to the peak level, and then gradually decreased. inhβb expression in testis maintained at a low level. During the embryonic developmental stages, inhα displayed the highest expression at 32-cell stage, whereas inhβb reached the highest expression at blastula stages. In situ hybridization data showed that both of inhα and inhβb were detected in oocytes of all stages. In male testis, inhα and inhβb was localized in spermatogonia, spermatocytes, spermatozoa, sertoli and leydig cells. In pseudomale testis, inhα showed the similar pattern in male testis, while the inhβb was detected in spermatocytes and spermatozoa. These data suggested that inhα may participate the spermatogenesis and oogenesis of Chinese tongue sole, while inhβb might predominantly function in oogenesis.
抑制素在脊椎动物的生殖和发育中发挥着重要作用。本研究克隆了中国舌鳎抑制素亚基 inhα 和 ihnβb 的两个基因。inhα 由 1032bp 组成，编码一个 343 个氨基酸的蛋白质。inhβb 由 1275bp 组成，编码一个 424 个氨基酸的蛋白质。系统进化树分析表明 INHα 和 INHβB 是独立进化的。qPCR 显示 inhα 在雄性睾丸中的表达高于卵巢和假雄睾丸，而 inhβb 在卵巢中的表达高于雄性和假雄睾丸。在性腺发育阶段，inhα 在卵巢和睾丸中的表达在孵化后 120 天（dph）达到最高，然后在卵巢中下降，但在睾丸中先下降后上升。同样，inhβb 在卵巢中的表达在 50-80 dph 时较低。在 120 dph 时，其表达显著增加到峰值水平，然后逐渐下降。inhβb 在睾丸中的表达保持在低水平。在胚胎发育阶段，inhα 在 32 细胞期表达最高，而 inhβb 在囊胚期表达最高。原位杂交数据显示 inhα 和 inhβb 在所有阶段的卵母细胞中均有检测到。在雄性睾丸中，inhα 和 inhβb 定位于精原细胞、精母细胞、精子、支持细胞和间质细胞。在假雄睾丸中，inhα 的表达模式与雄性睾丸相似，而 inhβb 则在精母细胞和精子中检测到。这些数据表明 inhα 可能参与中国舌鳎的精子发生和卵子发生，而 inhβb 可能主要在卵子发生中发挥作用。
