Kidsley Amanda K, White Rhys T, Beatson Scott A, Saputra Sugiyono, Schembri Mark A, Gordon David, Johnson James R, O'Dea Mark, Mollinger Joanne L, Abraham Sam, Trott Darren J
Australian Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia.
School of Chemistry and Molecular Biosciences, Australian Infectious Disease Research Centre, The University of Queensland, Brisbane, QLD, Australia.
Front Microbiol. 2020 Sep 2;11:1968. doi: 10.3389/fmicb.2020.01968. eCollection 2020.
sequence types 131 (ST131) and 1193 are multidrug-resistant extraintestinal pathogens that have recently spread epidemically among humans and are occasionally isolated from companion animals. This study characterized a nationwide collection of fluoroquinolone-resistant (FQ ) isolates from extraintestinal infections in Australian cats and dogs. For this, 59 cat and dog FQ clinical isolates (representing 6.9% of an 855-isolate collection) underwent PCR-based phylotyping and whole-genome sequencing (WGS). Isolates from commensal-associated phylogenetic groups A (14/59, 24%) and B1 (18/59, 31%) were dominant, with ST224 (10/59, 17%), and ST744 (8/59, 14%) predominating. Less prevalent were phylogenetic groups D (12/59, 20%), with ST38 (8/59, 14%) predominating, and virulence-associated phylogenetic group B2 (7/59, 12%), with ST131 predominating (6/7, 86%) and no ST1193 isolates identified. In a WGS-based comparison of 20 cat and dog-source ST131 isolates with 188 reference human and animal ST131 isolates, the cat and dog-source isolates were phylogenetically diverse. Although cat and dog-source ST131 isolates exhibited some minor sub-clustering, most were closely related to human-source ST131 strains. Furthermore, the prevalence of ST131 as a cause of FQ infections in Australian companion animals was relatively constant between this study and the 5-year-earlier study of Platell et al. (2010) (9/125 isolates, 7.2%). Thus, although the high degree of clonal commonality among FQ clinical isolates from humans vs. companion animals suggests the possibility of bi-directional between-species transmission, the much higher reported prevalence of ST131 and ST1193 among FQ clinical isolates from humans as compared to companion animals suggests that companion animals are spillover hosts rather than being a primary reservoir for these lineages.
序列类型131（ST131）和1193是耐多药的肠外病原体，最近在人类中流行传播，偶尔也从伴侣动物中分离出来。本研究对从澳大利亚猫和狗的肠外感染中分离出的耐氟喹诺酮（FQ）菌株进行了全国范围的特征分析。为此，对59株猫和狗的FQ临床分离株（占855株分离株集合的6.9%）进行了基于PCR的系统发育分型和全基因组测序（WGS）。来自共生相关系统发育组A（14/59，24%）和B1（18/59，31%）的分离株占主导地位，其中ST224（10/59，17%）和ST744（8/59，14%）占优势。系统发育组D（12/59，20%）较少见，其中ST38（8/59，14%）占优势，与毒力相关的系统发育组B2（7/59，12%）中，ST131占优势（6/7，86%），未鉴定出ST1193分离株。在基于WGS的20株猫和狗源ST131分离株与188株参考人和动物ST131分离株的比较中，猫和狗源分离株在系统发育上具有多样性。虽然猫和狗源ST131分离株表现出一些小的亚聚类，但大多数与人类源ST131菌株密切相关。此外，在本研究与Platell等人（2010年）5年前的研究中，ST131作为澳大利亚伴侣动物FQ感染原因的患病率相对恒定（9/125株分离株，7.2%）。因此，尽管来自人类与伴侣动物的FQ临床分离株之间高度的克隆共性表明存在种间双向传播的可能性，但与伴侣动物相比，人类FQ临床分离株中ST131和ST1193的报告患病率要高得多，这表明伴侣动物是溢出宿主，而不是这些谱系的主要储存宿主。
