Abdelbary Mohamed M H, Feil Edward J, Senn Laurence, Petignat Christiane, Prod'hom Guy, Schrenzel Jacques, François Patrice, Werner Guido, Layer Franziska, Strommenger Birgit, Pantosti Annalisa, Monaco Monica, Denis Olivier, Deplano Ariane, Grundmann Hajo, Blanc Dominique S
Service of Hospital Preventive Medicine, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.
Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH Aachen University Hospital, Aachen, Germany.
Front Microbiol. 2020 Aug 26;11:2063. doi: 10.3389/fmicb.2020.02063. eCollection 2020.
Methicillin-resistant (MRSA) is a common healthcare-associated pathogen that remains a major public health concern. Sequence type 228 (ST228) was first described in Germany and spread to become a successful MRSA clone in several European countries. In 2000, ST228 emerged in Lausanne and has subsequently caused several large outbreaks. Here, we describe the evolutionary history of this clone and identify the genetic changes underlying its expansion in Switzerland.
We aimed to understand the phylogeographic and demographic dynamics of MRSA ST228/ST111 by sequencing 530 representative isolates of this clone that were collected from 14 European countries between 1997 and 2012.
The phylogenetic analysis revealed distinct lineages of ST228 isolates associated with specific geographic origins. In contrast, isolates of ST111, which is a single locus variant of ST228 sharing the same type t041, formed a monophyletic cluster associated with multiple countries. The evidence points to a German origin of the sampled population, with the basal German lineage being characterized by type t001. The highly successful Swiss ST228 lineage diverged from this progenitor clone through the loss of the aminoglycoside-streptothricin resistance gene cluster and the gain of mupirocin resistance. This lineage was introduced first in Geneva and was subsequently introduced into Lausanne.
Our results reveal the radiation of distinct lineages of MRSA ST228 from a German progenitor, as the clone spread into different European countries. In Switzerland, ST228 was introduced first in Geneva and was subsequently introduced into Lausanne.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种常见的医疗保健相关病原体，仍然是主要的公共卫生问题。序列型228（ST228）首次在德国被描述，并传播至几个欧洲国家，成为一个成功的MRSA克隆株。2000年，ST228在洛桑出现，随后引发了几次大规模疫情。在此，我们描述了该克隆株的进化史，并确定了其在瑞士扩张的潜在基因变化。
我们旨在通过对1997年至2012年间从14个欧洲国家收集的530株该克隆株的代表性菌株进行测序，来了解MRSA ST228/ST111的系统地理学和种群动态。
系统发育分析揭示了与特定地理起源相关的ST228菌株的不同谱系。相比之下，ST111菌株是ST228的单一位点变体，具有相同的t041型，形成了一个与多个国家相关的单系簇。证据表明采样群体起源于德国，基础德国谱系的特征是t001型。高度成功的瑞士ST228谱系通过氨基糖苷类-链丝菌素抗性基因簇的缺失和莫匹罗星抗性的获得，从这个祖代克隆株分化而来。这个谱系首先在日内瓦被引入，随后被引入洛桑。
我们的结果揭示了随着该克隆株传播到不同欧洲国家，MRSA ST228的不同谱系从德国祖代辐射开来。在瑞士，ST228首先在日内瓦被引入，随后被引入洛桑。
Zotero 插件