Phuke Rahul Madhavrao, He Xinyao, Juliana Philomin, Bishnoi Santosh Kumar, Singh Gyanendra Pratap, Kabir Muhammad Rezaul, Roy Krishna Kanta, Joshi Arun Kumar, Singh Ravi Prakash, Singh Pawan Kumar
ICAR-Indian Agriculture Research Institute, Regional Station, Indore, India.
International Maize and Wheat Improvement Centre, Texcoco, Mexico.
Front Plant Sci. 2020 Aug 28;11:1309. doi: 10.3389/fpls.2020.01309. eCollection 2020.
Tan spot caused by () is an important disease of wheat in many wheat producing areas of the world. A genome wide association study (GWAS) was conducted using 11,401 SNP markers of the Illumina Infinium 15K Bead Chip with whole genome coverage to identify genomic regions associated with resistance to tan spot in a diverse panel of 184 wheat genotypes originating from South Asia and CIMMYT. The GWAS panel was phenotyped for seedling resistance to tan spot with race 1 in two greenhouse experiments. Besides CIMMYT germplasm, several lines from South Asia (India, Bangladesh and Nepal) showed good degree of resistance to tan spot. Association mapping was conducted separately for individual experiments and for pooled data using mixed linear model (MLM) and Fixed and random model Circulating Probability Unification (FarmCPU) model; no significant MTAs were recorded through the MLM model, whereas FarmCPU model reported nine significant MTAs located on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6A, and 7D. The long arms of chromosomes 5A and 5B were consistent across both environments, in which the locus was found in identified region of chromosome 5A, and MTA at IACX9261 on 5BL appears to represent the resistance gene . MTAs observed on chromosomes 1B, 2A, 2B, 3B, 4A, 6A, and 7D have not been reported previously and are likely novel.
由（）引起的黄斑叶枯病是世界上许多小麦产区的一种重要小麦病害。利用Illumina Infinium 15K芯片具有全基因组覆盖的11401个单核苷酸多态性（SNP）标记，对来自南亚和国际玉米小麦改良中心（CIMMYT）的184个不同小麦基因型组成的群体进行全基因组关联研究（GWAS），以鉴定与抗黄斑叶枯病相关的基因组区域。在两个温室试验中，对该GWAS群体进行了对1号生理小种黄斑叶枯病的苗期抗性表型分析。除了CIMMYT种质外，南亚（印度、孟加拉国和尼泊尔）的几个品系对黄斑叶枯病表现出良好的抗性程度。分别对单个试验和合并数据使用混合线性模型（MLM）和固定与随机模型循环概率统一（FarmCPU）模型进行关联作图；通过MLM模型未记录到显著的标记-性状关联（MTA），而FarmCPU模型报告了位于1B、2A、2B、3B、4A、5A、5B、6A和7D染色体上的9个显著MTA。5A和5B染色体的长臂在两种环境下是一致的，其中在5A染色体的鉴定区域发现了该位点，5BL上的IACX9261处的MTA似乎代表抗性基因。在1B、2A、2B、3B、4A、6A和7D染色体上观察到的MTA以前未见报道，可能是新的。
