Otsuka H, Yoshida K, Kusano S, Kashimura M, Okamura Y
Nihon Sanka Fujinka Gakkai Zasshi. 1987 May;39(5):746-50.
To elucidate the pathogenesis of leiomyoma, myoma tissues and myoma-derived cultured cells were stained with desmin, vimentin and fibronectin by the enzyme-labelled antibody method. Desmin staining was positive in both the myoma tissues and the cultured cells. Vimentin staining was positive in endothelial cells in the blood vessels of the myoma and in the cultured cells. Fibronectin staining was positive in both the myoma and cultured cells. These findings demonstrate that the leiomyoma was derived from myogenic cells. It was suggested that the myoma-derived cultured cells were myofibroblasts with myoblasts and fibroblasts, and had the ability to differentiate. It appears that the original cells of the myoma differentiate to myofibroblasts, which then differentiate into myoblasts and fibroblasts.
为阐明平滑肌瘤的发病机制，采用酶标抗体法对肌瘤组织及肌瘤来源的培养细胞进行结蛋白、波形蛋白和纤连蛋白染色。结蛋白染色在肌瘤组织和培养细胞中均呈阳性。波形蛋白染色在肌瘤血管内皮细胞及培养细胞中呈阳性。纤连蛋白染色在肌瘤及培养细胞中均呈阳性。这些结果表明平滑肌瘤起源于肌源性细胞。提示肌瘤来源的培养细胞是具有成肌细胞和平滑肌细胞特征的肌成纤维细胞，且具有分化能力。看来肌瘤的原始细胞先分化为肌成纤维细胞，然后再分化为成肌细胞和平滑肌细胞。
