Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R. China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, P.R. China; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, P.R. China.
Poult Sci. 2020 Oct;99(10):5149-5157. doi: 10.1016/j.psj.2020.06.040. Epub 2020 Jul 3.
Improvac has been tentatively used to immune-castrate roosters. The aim of this study was to investigate whether Improvac affected skeletal muscle development in chickens. The muscle fiber type and size and the expression levels of genes related to muscle development in pectoral and thigh muscles were examined at 5, 9, and 14 wk of age in the control, early, late, and early + late Improvac-treated groups. Immunocastration with Improvac affected the development of thigh muscles and the expression of MYH1B, MSTN, and SM. The cross-sectional area in the early group was significantly larger than in the control group at the 14th week (P < 0.01). At the fifth week, the expression levels of MYH1B, MYOD, and MSTN in the early group were significantly higher than those in the control group (P < 0.05), and at the ninth week, the expression level of SM1 in the control group was significantly lower than that in early and late groups (P < 0.05). Immunocastration did not affect pectoral muscle development or the expression of genes related to muscle development.
已初步尝试使用 Improvac 对公鸡进行免疫去势。本研究旨在探讨 Improvac 是否会影响鸡的骨骼肌发育。在 5、9 和 14 周龄时，分别对对照组、早期组、晚期组和早期+晚期 Improvac 处理组的胸肌和腿肌中肌纤维类型和大小以及与肌肉发育相关的基因表达水平进行了检测。使用 Improvac 进行免疫去势会影响腿肌的发育和 MYH1B、MSTN 和 SM 的表达。第 14 周时，早期组的横截面积明显大于对照组（P<0.01）。第 5 周时，早期组的 MYH1B、MYOD 和 MSTN 表达水平明显高于对照组（P<0.05），第 9 周时，对照组的 SM1 表达水平明显低于早期和晚期组（P<0.05）。免疫去势对胸肌发育或与肌肉发育相关的基因表达没有影响。
