Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Islamic Republic of Iran.
Scientific Information and Communication Department, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Islamic Republic of Iran.
Curr Microbiol. 2020 Dec;77(12):3919-3926. doi: 10.1007/s00284-020-02222-w. Epub 2020 Sep 28.
The emergence of diseases has caused much health and economic damage. Viral Nervous Necrosis (VNN) is considered as one of the most important threats to aquatic ecosystems. VNN can cause severe mortality and economic loss in fish farms. The high water temperatures in southern Iran and the observed incidences of fish mortality in the Persian Gulf led to the hypothesis of the possible emergence of VNN. Therefore, this study aimed to monitor two species of fish susceptible to VNN using PCR, and Nested PCR methods and comparing the sensitivity of these methods to the identification of Betanodavirus infection in apparently healthy and symptomatic fish. About 850 Grouper (Epinephelus spp.) and Asian Sea bass (Lates calcarifer) fish of the Persian Gulf were collected randomly and examined. Molecular methods were used to identify NNV in visibly healthy and symptomatic fish of the Persian Gulf of Iran. The results of the PCR showed no positive cases, but the Nested PCR revealed some positive results. Then, the phylogenetic analysis of the virus sequence was performed. The nucleotide sequence of Nested PCR products revealed a 98-100% homology with Red Spotted Grouper Viral Nervous Necrosis (RGNNV). This is the first report on VNN tracing and detection as well as phylogenetic analysis of the virus from the Persian Gulf of Iran. Therefore, considering the importance of emerging viral diseases and the irreparable damage they cause, continuous monitoring and epidemiological studies of VNN were recommended by authorized organizations.
疾病的出现给健康和经济造成了很大的破坏。病毒性神经坏死病（VNN）被认为是对水生生态系统的最重要威胁之一。VNN可导致鱼类养殖场严重死亡和经济损失。伊朗南部的高水温以及波斯湾观察到的鱼类死亡事件导致了 VNN 可能出现的假设。因此，本研究旨在使用 PCR 和巢式 PCR 方法监测两种易感染 VNN 的鱼类，并比较这些方法对鉴定明显健康和有症状鱼类中 Betanodavirus 感染的敏感性。随机采集了约 850 尾波斯湾的石斑鱼（Epinephelus spp.）和亚洲海鲈（Lates calcarifer）并进行了检查。分子方法用于鉴定伊朗波斯湾可见健康和有症状鱼类中的 NNV。PCR 结果没有呈阳性的病例，但巢式 PCR 显示了一些阳性结果。然后，对病毒序列进行了系统发育分析。巢式 PCR 产物的核苷酸序列与红鳍石斑鱼病毒性神经坏死病（RGNNV）具有 98-100%的同源性。这是首次从伊朗波斯湾报道 VNN 的追踪和检测以及病毒的系统发育分析。因此，考虑到新兴病毒性疾病的重要性及其造成的不可挽回的损害，建议授权组织对 VNN 进行持续监测和流行病学研究。
