Department of Stomatology, Chinese PLA General Hospital, No.28 FuXing Road, Beijing, 100853, People's Republic of China.
Medical Supplier Center, Department of Pharmacy, Chinese PLA General Hospital, Beijing, 100853, China.
Mol Cell Biochem. 2021 Jan;476(1):303-310. doi: 10.1007/s11010-020-03907-3. Epub 2020 Sep 29.
Although tooth loss is a known risk factor of cognitive function, whether and how the chewing-side preference (CSP) affects memory impairment still remains unclear. This study evaluates the behavior changes in mice after the loss of teeth on one side and explores the role of serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) signal pathway within these changes. To this end, CSP mouse models with either the removal of left unilateral molars (CSP-L) or right unilateral molars (CSP-R) were established. Morris water maze test and passive avoidance test were performed to evaluate the mice's learning and memory capacity in the 4th and 8th weeks. The correlation between CSP and brain function changes was validated with changes in 5-HT and BDNF levels. CSP mice's cognitive function was found to be decreased, along with a significant decline in 5-HT1A level, especially in CSP-R mice. BDNF and TrkB levels in CSP-R mice were also significantly lowered. These findings suggest that CSP results in memory impairment, which is associated with the 5-HT-BDNF signaling pathway.
虽然牙齿缺失是认知功能下降的已知风险因素，但咀嚼侧偏好（CSP）是否以及如何影响记忆障碍仍不清楚。本研究评估了单侧牙齿缺失后小鼠的行为变化，并探讨了 5-羟色胺（5-HT）和脑源性神经营养因子（BDNF）信号通路在此变化中的作用。为此，建立了单侧磨牙去除的 CSP 小鼠模型（CSP-L）或右侧单侧磨牙去除的 CSP 小鼠模型（CSP-R）。在第 4 周和第 8 周进行 Morris 水迷宫试验和被动回避试验，以评估小鼠的学习和记忆能力。通过 5-HT 和 BDNF 水平的变化验证 CSP 与脑功能变化之间的相关性。发现 CSP 小鼠的认知功能下降，5-HT1A 水平显著降低，尤其是 CSP-R 小鼠。CSP-R 小鼠的 BDNF 和 TrkB 水平也明显降低。这些发现表明，CSP 导致记忆障碍，这与 5-HT-BDNF 信号通路有关。
