多囊卵巢综合征少女肠道微生物群：随机治疗的影响。

Gut microbiota in adolescent girls with polycystic ovary syndrome: Effects of randomized treatments.

机构信息

Endocrinology Department, Pediatric Research Institute Hospital Sant Joan de Déu, University of Barcelona, Barcelona, Spain.

Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), ISCIII, Madrid, Spain.

出版信息

Pediatr Obes. 2021 Apr;16(4):e12734. doi: 10.1111/ijpo.12734. Epub 2020 Sep 29.

DOI:10.1111/ijpo.12734

PMID:32989872

Abstract

BACKGROUND

Girls with obesity and polycystic ovary syndrome (PCOS) and women with PCOS have altered gut microbiota.

OBJECTIVE

To study the gut microbiota composition of girls with PCOS without obesity (age, 15.8 years; body mass index [BMI] 25 kg/m ) and the effects of randomized treatments with an oral contraceptive (OC, N = 15) or with spironolactone-pioglitazone-metformin (SPIOMET, N = 15) for 1 year. Thirty-one age-matched girls served as controls.

METHODS

16S ribosomal subunit gene amplicon sequencing was performed in stool samples from all subjects; samples from 23 out of 30 girls with PCOS (OC, N = 12; SPIOMET, N = 11) were available for analysis post-treatment. Clinical and endocrine-metabolic variables were measured before and after intervention.

RESULTS

Girls with PCOS had decreased diversity alpha, altered microbiota pattern and taxonomic profile with more abundance of Family XI (P = .002), and less abundance of family Prevotellaceae (P = .0006) the genus Prevotella (P = .0001) and Senegalimassilia (P < .0001), as compared to controls. Family XI abundance related positively to hepato-visceral fat (R = 0.453; P = .0003). SPIOMET treatment, but not OC, normalized the abundance of Family XI. Prevotellaceae, Prevotella and Senegalimassilia abundance remained unchanged after either treatment.

CONCLUSION

SPIOMET's spectrum of normalizing effects in girls with PCOS is herewith broadened as to include Family XI abundance in gut microbiota.

摘要

背景

患有肥胖症和多囊卵巢综合征（PCOS）的女孩以及患有 PCOS 的女性的肠道微生物群发生了改变。

目的

研究无肥胖的 PCOS 女孩（年龄为 15.8 岁；体重指数 [BMI] 为 25kg/m ）的肠道微生物群组成，以及接受为期 1 年的口服避孕药（OC）或螺内酯-吡格列酮-二甲双胍（SPIOMET）随机治疗的影响。31 名年龄匹配的女孩作为对照组。

方法

对所有受试者的粪便样本进行 16S 核糖体亚基基因扩增子测序；23 名 PCOS 女孩（OC，N=12；SPIOMET，N=11）中有 12 名的样本可用于治疗后分析。在干预前后测量临床和内分泌代谢变量。

结果

与对照组相比，患有 PCOS 的女孩的多样性 alpha 降低，微生物群模式和分类群特征发生改变，XI 科（Family XI）的丰度增加（P=0.002），而 Prevotellaceae 科（Family Prevotellaceae）、Prevotella 属（Genus Prevotella）和 Senegalimassilia 属（Genus Senegalimassilia）的丰度降低（P=0.0006、0.0001 和 <0.0001）。XI 科的丰度与肝内脏脂肪呈正相关（R=0.453；P=0.0003）。SPIOMET 治疗而非 OC 治疗可使 XI 科的丰度正常化。两种治疗后，Prevotellaceae、Prevotella 和 Senegalimassilia 的丰度均无变化。