Older Caitlin E, Diesel Alison B, Starks Jill M, Lawhon Sara D, Rodrigues Hoffmann Aline
Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Vet Dermatol. 2021 Feb;32(1):61-e10. doi: 10.1111/vde.12885. Epub 2020 Sep 29.
Various Staphylococcus species have been demonstrated to play important roles on the skin, including causing disease and protecting the host from pathogens. Although culture-based studies have isolated various Staphylococcus spp. from feline skin, very little is known regarding the species-level communities on the host.
HYPOTHESIS/OBJECTIVES: To describe the species-level staphylococcal communities inhabiting the skin of healthy cats and cats with allergic dermatitis.
Skin swabs from the ear canal and groin of 11 healthy and 10 allergic (nonlesional) cats were obtained.
DNA was extracted from the skin swabs and used for next-generation sequencing targeting the V1-3 region of the 16S rRNA gene. Following a standard microbiota analysis of the sequencing data, species-level assignment for the staphylococcal sequences were obtained using a staphylococci-specific database.
Staphylococcus spp. had similar relative abundance in healthy and allergic samples. The most abundant staphylococcal species were S. epidermidis in healthy samples, and S. felis and S. capitis in allergic samples. The composition of staphylococcal communities, as well as relative abundance of Staphylococcus spp., was variable between body sites and individual cats sampled.
These results demonstrate that diverse staphylococcal communities inhabit the skin of healthy and allergic cats, and provide a starting point for further research into the importance of Staphylococcus spp. in feline allergic skin disease.
多种葡萄球菌已被证明在皮肤上发挥重要作用，包括引发疾病以及保护宿主免受病原体侵害。尽管基于培养的研究已从猫的皮肤中分离出多种葡萄球菌，但对于宿主上物种水平的群落了解甚少。
假设/目标：描述健康猫和患有过敏性皮炎的猫皮肤上的葡萄球菌物种水平群落。
采集了11只健康猫和10只患有过敏性（非病变）猫的耳道和腹股沟皮肤拭子。
从皮肤拭子中提取DNA，并用于靶向16S rRNA基因V1 - 3区域的下一代测序。在对测序数据进行标准微生物群分析后，使用葡萄球菌特异性数据库获得葡萄球菌序列的物种水平分类。
葡萄球菌在健康样本和过敏样本中的相对丰度相似。健康样本中最丰富的葡萄球菌物种是表皮葡萄球菌，过敏样本中是猫葡萄球菌和头葡萄球菌。葡萄球菌群落的组成以及葡萄球菌物种的相对丰度在不同身体部位和所采样的个体猫之间存在差异。
这些结果表明，健康猫和过敏猫的皮肤上栖息着多样的葡萄球菌群落，并为进一步研究葡萄球菌在猫过敏性皮肤病中的重要性提供了一个起点。
