Department of Internal Medicine, Hacettepe University School of Medicine, Ankara, Turkey.
Department of Pharmaceutical Microbiology, Hacettepe University Faculty of Pharmacy, Ankara, Turkey.
Eur J Clin Invest. 2021 Apr;51(4):e13417. doi: 10.1111/eci.13417. Epub 2020 Oct 14.
Limited available animal and human data suggest an association between dysbiosis of gut microbiota and PCOS. We aimed to determine whether gut microbiota in lean women with PCOS shows any alterations compared to healthy women.
Twenty-four lean patients with PCOS phenotype A according to the Rotterdam 2003 diagnostic criteria and 22 BMI-matched healthy women were included in this study. Anthropometric, hormonal and biochemical measurements were carried out in all participants. 16S rRNA gene V3-V4 region amplicon sequencing was performed on stool samples. Preprocessing of the raw data was performed using QIIME, and both QIIME and R packages were used for microbiome analysis.
Bacterial richness and diversity did not show a significant difference between patients and controls. Beta diversity was similar between the groups. However, Erysipelotrichaceae, Proteobacteria, Gammaproteobacteria, Enterobacteriaceae, Planococcaceae, Gemmules and Bacillales were significantly abundant in PCOS group according to LEfSe analysis. Clostridium cluster XVII showed increased abundance in patient group, while Clostridium sensustricto and Roseburia were decreased compared to controls. Random forest prediction analysis revealed Clostridium cluster XIVb as the most discriminative feature of patient group and Roseburia for healthy controls. Testosterone and androstenedione were negatively correlated with alpha and phylogenetic diversity.
Our results suggest that gut microbiome of lean PCOS patients with full phenotype shows compositional alterations with similar bacterial richness and diversity compared to controls and that hyperandrogenism is associated with dysbiosis.
有限的动物和人类数据表明，肠道微生物群落失调与 PCOS 之间存在关联。我们旨在确定瘦型 PCOS 患者的肠道微生物群与健康女性相比是否存在任何改变。
本研究纳入了 24 名符合 2003 年鹿特丹诊断标准的 A 型 PCOS 表型的瘦型患者和 22 名 BMI 匹配的健康女性。所有参与者均进行了人体测量、激素和生化测量。对粪便样本进行 16S rRNA 基因 V3-V4 区扩增子测序。使用 QIIME 对原始数据进行预处理，使用 QIIME 和 R 包进行微生物组分析。
患者和对照组的细菌丰富度和多样性没有显著差异。两组之间的β多样性相似。然而，根据 LEfSe 分析，厚壁菌门、变形菌门、γ变形菌门、肠杆菌科、棒状杆菌科、Gemmules 和芽孢杆菌目在 PCOS 组中明显丰富。梭菌簇 XVII 在患者组中丰度增加，而梭菌 sensustricto 和罗氏菌则比对照组减少。随机森林预测分析显示，梭菌簇 XIVb 是患者组最具鉴别特征的特征，而罗氏菌是健康对照组的特征。睾酮和雄烯二酮与 alpha 和系统发育多样性呈负相关。
我们的结果表明，具有完整表型的瘦型 PCOS 患者的肠道微生物群与对照组相比表现出组成上的改变，具有相似的细菌丰富度和多样性，并且高雄激素血症与菌群失调有关。
