School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
Discipline of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
J Ethnopharmacol. 2021 Feb 10;266:113418. doi: 10.1016/j.jep.2020.113418. Epub 2020 Sep 28.
Danshen, is a traditional Chinese medicine obtained from the dried root and rhizome of Salvia miltiorrhiza Bunge. It is known to be used for neurological disorder including for Alzheimer's disease (AD). This study uncovers the effect of Danshen water extract on the Alzheimer's disease model of C.elegans.
The composition of Danshen water extract was determined using (High Performance Liquid Chromatography (HPLC). Then Thioflavin T assay was used to determined if Danshen water extract could prevent the aggregation of amyloid-β peptide (Aβ). Alzheimer's disease C.elegans model was used to determine the effect of Danshen water extract. Finally, the reactive oxygen species (ROS) was determined using the 2,7-dichlorofuorescein diacetate method.
In this study, we found that standardized Danshen water extract that contains danshensu (1.26%), salvianolic acid A (0.35%) and salvianolic acid B (2.21%) are able to bind directly to Aβ and prevents it from aggregating. The IC for the inhibition of Aβ aggregation by Danshen water extract was 0.5 mg/ml. In the AD model of C.elegans, Danshen water extract managed to alleviates the paralysis phenotype. Furthermore, the administration of Danshen water extract displayed antioxidant properties toward the Aβ-induced oxidative stress.
AD is a widespread neurodegenerative disease attributed to the accumulation of extracellular plaques comprising Aβ. Danshen water extract could significantly reduce the progress of paralysis in the AD model of C. elegans, showing promising results with its antioxidant properties. It can be concluded that Danshen water extract could potentially serve as a therapeutic for AD.
丹参是一种传统的中药，由丹参的干燥根和根茎制成。已知它可用于治疗包括阿尔茨海默病（AD）在内的神经紊乱。本研究揭示了丹参水提取物对秀丽隐杆线虫 AD 模型的作用。
使用（高效液相色谱法（HPLC））测定丹参水提取物的组成。然后使用硫代黄素 T 测定法确定丹参水提取物是否可以防止淀粉样β肽（Aβ）的聚集。使用阿尔茨海默病秀丽隐杆线虫模型来确定丹参水提取物的作用。最后，使用 2,7-二氯荧光素二乙酸酯法测定活性氧（ROS）。
在这项研究中，我们发现含有丹参素（1.26%）、丹酚酸 A（0.35%）和丹酚酸 B（2.21%）的标准化丹参水提取物能够直接与 Aβ结合并阻止其聚集。丹参水提取物抑制 Aβ聚集的 IC 为 0.5mg/ml。在秀丽隐杆线虫的 AD 模型中，丹参水提取物能够缓解瘫痪表型。此外，丹参水提取物的给药表现出对 Aβ诱导的氧化应激的抗氧化特性。
AD 是一种广泛存在的神经退行性疾病，归因于包含 Aβ 的细胞外斑块的积累。丹参水提取物可显著减缓 AD 模型中秀丽隐杆线虫的瘫痪进展，其抗氧化特性显示出有希望的结果。可以得出结论，丹参水提取物可能作为 AD 的治疗方法。
