Elaini Rachid, Asadi Romisa, Naish Neil, Koukidou Martha, Ahmed Mazih
Hassan II Institute of Agronomy and Veterinary Medicine, Complexe Horticole d'Agadir, Ait Melloul 80150, Morocco.
Omnium Agricole du Souss, Tassilla III, Agadir 80000, Morocco.
Insects. 2020 Sep 27;11(10):663. doi: 10.3390/insects11100663.
The Mediterranean fruit fly (medfly), , is a significant pest of stone and pome fruit that causes considerable economic losses worldwide. Current control is primarily based on insecticides, which are often mixed with protein baits. Oxitec has developed a self-limiting medfly strain (OX3864A) that demonstrates conditional female-specific mortality in the early life stages. Sustained release of OX3864A males offers medfly control, which should lead to substantial economic benefits in area-wide programmes. In the current study, the optimum quantities of mature and immature stages of the strain are assessed under semi-mass production. Moreover, the rearing and quality control limitations related to the production of this strain are provided. The data here demonstrate that the egg hatch rate can reach >85% under optimum rearing conditions. However, this depends on the number of pupae loaded in a cage and their ages. The suggested pupal density ranges between 14,000 and 18,000 pupae per cage to provide optimum egg production. In parallel, the embryo densities of 1.25-1.5 mL/kg larval Tet+ diet are recommended for strain propagation, while embryo densities of 1.25-2.0 mL/kg larval Tet- diet are suggested for male-only pupal production.
地中海实蝇是核果和梨果类水果的一种重要害虫，在全球范围内造成了相当大的经济损失。目前的防治主要基于杀虫剂，杀虫剂通常与蛋白质诱饵混合使用。Oxitec公司开发了一种自我限制的地中海实蝇品系（OX3864A），该品系在生命早期阶段表现出有条件的雌性特异性死亡。持续释放OX3864A雄蝇可防治地中海实蝇，这将在区域防治计划中带来可观的经济效益。在本研究中，在半大规模生产条件下评估了该品系成熟和未成熟阶段的最佳数量。此外，还提供了与该品系生产相关的饲养和质量控制方面的限制。此处的数据表明，在最佳饲养条件下，卵孵化率可达到85%以上。然而，这取决于笼子中装入的蛹的数量及其年龄。建议每个笼子的蛹密度在14000至18000个之间，以实现最佳的产卵量。同时，对于品系繁殖，建议幼虫Tet+饲料的胚胎密度为1.25 - 1.5 mL/kg，而对于仅生产雄蛹，建议幼虫Tet-饲料的胚胎密度为1.25 - 2.0 mL/kg。
