State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Genes (Basel). 2020 Sep 27;11(10):1136. doi: 10.3390/genes11101136.
Emerging evidence indicates that the host microRNAs (miRNAs) are important intracellular regulators and play pivotal roles in intricate host-pathogen interaction networks. In our previous studies, ssc-microRNA-4334-5p (miR-4334-5p) was identified as a differentially expressed miRNA in microarray-based miRNAs profiling experiment, but whether miR-4334-5p regulates foot and mouth disease virus (FMDV) propagation is less understood. Here, we demonstrated that miR-4334-5p expression level was up-regulated shortly after FMDV infection, transfection of miR-4334-5p mimics promoted, while inhibitor transfection suppressed FMDV replication correspondingly. Further bioinformatic analysis and experimental study suggested ID1 was the direct target of miR-4334-5p, suppressing FMDV replication by regulating interferon (IFN) pathways. These findings shed light on microRNAs-ID1-interferon axis in regulating FMDV replication.
新出现的证据表明，宿主 microRNAs（miRNAs）是重要的细胞内调节剂，在复杂的宿主-病原体相互作用网络中发挥关键作用。在我们之前的研究中，ssc-microRNA-4334-5p（miR-4334-5p）被鉴定为基于微阵列的 miRNAs 分析实验中差异表达的 miRNA，但 miR-4334-5p 是否调节口蹄疫病毒（FMDV）的传播尚不清楚。在这里，我们证明了 miR-4334-5p 的表达水平在 FMDV 感染后不久就上调了，转染 miR-4334-5p 模拟物促进了 FMDV 的复制，而抑制剂转染则相应地抑制了 FMDV 的复制。进一步的生物信息学分析和实验研究表明，ID1 是 miR-4334-5p 的直接靶标，通过调节干扰素（IFN）途径来抑制 FMDV 的复制。这些发现揭示了 microRNAs-ID1-干扰素轴在调节 FMDV 复制中的作用。
