Department of Surgical Oncology, Medical University of Lublin, Radziwiłłowska 13 St., 20-080 Lublin, Poland.
Department of Human Physiology, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, Poland.
Cells. 2020 Sep 25;9(10):2168. doi: 10.3390/cells9102168.
Cytokeratin-19 (CK19) has been proven to be commonly expressed by cancer cells in a variety of solid tumors and may serve as a suitable marker of metastases in gastric cancer (GC). Since objective assessment of peritoneal lavage or fluid for free cancer cells (FCC) is essential for clinical decision making in patients with GC, it is important to develop a quantitative and reproducible method for such evaluation. We assessed the possible application of One-Step Nucleic Acid amplification (OSNA) assay as a rapid method for FCC detection in intraoperative peritoneal lavage or fluid of GC patients. Seventy-eight intraoperative peritoneal lavage or fluid samples were eligible for the analysis by conventional cytology and OSNA examination. The concentration of CK19 mRNA in intraoperative peritoneal lavage and fluid was compared with the conventional cytological assessment. CK19 mRNA concentration was detected by OSNA assay. For peritoneal lavage samples, sensitivity and specificity were 83.3% and 87.8%, respectively. In peritoneal fluid, significantly higher CK19 values were observed in patients with serosal infiltration (medians: 100 copies/µL vs. 415.7 copies/µL; = 0.0335) and lymph node metastases (medians: 2.48 copies/µL vs. 334.8 copies/µL). OSNA assay turns out to be an objective, fast, and reproducible quantitative method of FCC assessment.
细胞角蛋白 19（CK19）已被证明在多种实体瘤的癌细胞中广泛表达，可能作为胃癌（GC）转移的合适标志物。由于客观评估胃癌患者腹腔灌洗液或游离癌细胞（FCC）对于临床决策至关重要，因此开发一种用于此类评估的定量和可重复的方法非常重要。我们评估了一步法核酸扩增（OSNA）检测作为 GC 患者术中腹腔灌洗或腹腔液中 FCC 快速检测的可能应用。对 78 例术中腹腔灌洗或腹腔液进行了常规细胞学和 OSNA 检查的分析。比较了术中腹腔灌洗液和腹腔液中 CK19 mRNA 的浓度与常规细胞学评估的结果。采用 OSNA 法检测 CK19 mRNA。对于腹腔灌洗样本，OSNA 的敏感性和特异性分别为 83.3%和 87.8%。在腹腔液中，浆膜浸润患者的 CK19 值明显更高（中位数：100 拷贝/μL 比 415.7 拷贝/μL；=0.0335），淋巴结转移患者的 CK19 值明显更高（中位数：2.48 拷贝/μL 比 334.8 拷贝/μL）。OSNA 检测是一种客观、快速、可重复的 FCC 定量评估方法。
