Kamar Samaa Samir, Latif Noha Samir Abdel, Elrefai Mohamed Fathi Mohamed, Amin Shaimaa Nasr
Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Anat Cell Biol. 2020 Sep 30;53(3):301-312. doi: 10.5115/acb.20.055.
Gastric ulcer is one of the most serious diseases. Nebivolol (Neb), a β1-blocker, exhibits vasodilator and anti-oxidative properties, simvastatin (Sim) antihyperlipidemic drug, exhibits anti-oxidative, anti-inflammatory properties and promote endogenous nitric oxide (NO) production. The aim of this study was to evaluate the gastroprotective effects of Neb and Sim against cold restraint stress (CRS)-induced gastric ulcer in rats. Rats were restrained, and maintained at 4°C for 3 hours. Animals were divided into six groups; control group, CRS group, and four treatment groups received ranitidine (Ran), Neb, Sim and both Neb and Sim. Treatments were given orally on a daily basis for 7 days prior to CRS. The gastroprotective effects of Neb and Sim were assessed biochemically by measuring variations in prostaglandins E2, NO, reduced glutathione and malondialdehyde, and functionally by estimating force of contractions of isolated rat fundus in the studied groups in response to acetylecholine stimulation and morphologically using hematoxylin and eosin staining, periodic acid Schiff's reaction and immunohistochemistry for cyclooxygenase 2 in gastric mucosa. CRS caused significant ulcerogenic effect. Alternatively, pretreatment with Ran, Neb, and Sim significantly corrected biochemical findings, pharmacological and histological studies.
胃溃疡是最严重的疾病之一。奈必洛尔（Neb）是一种β1受体阻滞剂，具有血管舒张和抗氧化特性；辛伐他汀（Sim）是一种抗高血脂药物，具有抗氧化、抗炎特性并能促进内源性一氧化氮（NO）生成。本研究的目的是评估奈必洛尔和辛伐他汀对大鼠冷束缚应激（CRS）诱导的胃溃疡的胃保护作用。将大鼠束缚，并在4°C下维持3小时。动物分为六组：对照组、CRS组以及四个治疗组，分别给予雷尼替丁（Ran）、奈必洛尔、辛伐他汀以及奈必洛尔和辛伐他汀联合用药。在CRS前连续7天每日口服给药。通过测量前列腺素E2、NO、还原型谷胱甘肽和丙二醛的变化，从生化方面评估奈必洛尔和辛伐他汀的胃保护作用；通过估计研究组中分离的大鼠胃底对乙酰胆碱刺激的收缩力，从功能方面进行评估；并使用苏木精和伊红染色、过碘酸希夫反应以及胃黏膜中环氧化酶2的免疫组织化学，从形态学方面进行评估。CRS引起了显著的致溃疡作用。相反，用雷尼替丁、奈必洛尔和辛伐他汀预处理显著纠正了生化结果、药理学和组织学研究结果。
