Elsaed Wael M, Alahmadi Abdulaziz M, Al-Ahmadi Basil T, Taha Jumana A, Tarabishi Raghad M
Anatomy & Embryology Department, Taibah University, Almadinah Almunawwarah, KSA.
Anatomy & Embryology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Taibah Univ Med Sci. 2018 May 31;13(5):422-431. doi: 10.1016/j.jtumed.2018.04.010. eCollection 2018 Oct.
Stress-induced peptic ulcer disease (SPUD) refers to erosions in the mucosa of the upper gastrointestinal tract that are caused by stress. Some antidepressants are reported to have antioxidant and antiulcer effects. However, histopathological and biochemical evaluation of the anti-ulcer activity of a comparable antidepressant, fluvoxamine, has not been adequately investigated. This study aims to determine the anti-ulcer efficacy of fluvoxamine in reducing stress-induced histopathological and biochemical changes in the gastric mucosa.
Thirty adult male albino rats were divided into three groups of 10 rats each: the control groups, the SPUD group, and the fluvoxamine-pre-treated group, which received fluvoxamine for eight days before stress exposure. The cold-restraint stress method was used to induce stomach ulcers in the SPUD and fluvoxamine groups. Afterward, the stomachs of rats were removed, opened, and ulcer indices were calculated. Light microscopy was performed following haematoxylin and eosin staining, periodic acid Schiff's, Masson's trichrome staining, and proliferating cell nuclear antigen immunostaining. Gastric tissue levels of oxidative stress markers were measured and compared among groups.
The stomachs of the fluvoxamine-treated rats showed a significantly lower number of ulcers with minimal mucosal injury compared with those of rats from the SPUD group. The oxidative stress marker levels and SPUD ulcer indices were significantly different among groups.
Fluvoxamine pre-treatment exerted a gastroprotective effect against ulcer development and promoted healing of the developed lesions.
应激性消化性溃疡病（SPUD）是指由应激引起的上消化道黏膜糜烂。据报道，一些抗抑郁药具有抗氧化和抗溃疡作用。然而，对于一种类似的抗抑郁药氟伏沙明的抗溃疡活性，尚未进行充分的组织病理学和生化评估。本研究旨在确定氟伏沙明在减轻应激引起的胃黏膜组织病理学和生化变化方面的抗溃疡疗效。
30只成年雄性白化大鼠分为三组，每组10只：对照组、SPUD组和氟伏沙明预处理组，后者在应激暴露前8天接受氟伏沙明治疗。采用冷束缚应激法在SPUD组和氟伏沙明组诱导胃溃疡。之后，取出大鼠的胃，打开并计算溃疡指数。苏木精和伊红染色、过碘酸希夫氏染色、马松三色染色以及增殖细胞核抗原免疫染色后进行光镜检查。测量并比较各组胃组织氧化应激标志物水平。
与SPUD组大鼠相比，氟伏沙明治疗的大鼠胃中溃疡数量显著减少，黏膜损伤最小。各组间氧化应激标志物水平和SPUD溃疡指数存在显著差异。
氟伏沙明预处理对溃疡发展具有胃保护作用，并促进已形成病变的愈合。
