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基于肠道微生态的古法“金汁”成分

Composition of "gold juice" using an ancient method based on intestinal microecology.

作者信息

Li Qiuwei, Guo Liying, Wang Li, Miao Jing, Cui Huantian, Li Li, Geng Kan, Zhao Licong, Sun Xiaoxue, Jia Jianwei, Bian Yuhong

机构信息

Tianjin Second People's Hospital, Tianjin, China.

Integrative Medicine Institute, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

出版信息

J Int Med Res. 2020 Sep;48(9):300060520931288. doi: 10.1177/0300060520931288.

DOI:10.1177/0300060520931288
PMID:32993381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7545780/
Abstract

OBJECTIVE

To identify potentially effective bacterial components of gold juice, a traditional Chinese medicine treatment used for fecal microbiota transplantation.

METHODS

Fecal samples were collected from five healthy children (two boys and three girls; mean age, 7.52 ± 2.31 years). The children had no history of antibiotic use or intestinal microecological preparation in the preceding 3 months. Fresh fecal samples were collected from children to prepare gold juice in mid-to-late November, in accordance with traditional Chinese medicine methods, then used within 7 days. Finally, 16S rDNA sequence analysis was used to identify potentially effective bacterial components of gold juice. QIIME software was used for comparisons of microbial species among gold juice, diluent, filtrate, and loess samples.

RESULTS

Microflora of gold juice exhibited considerable changes following "ancient method" processing. Microbial components significantly differed between gold juice and filtrate samples. The gold juice analyzed in our study consisted of microbes that synthesize carbohydrates and amino acids by degrading substances, whereas the filtrate contained probiotic flora, , and 9.

CONCLUSIONS

This study of microbial components in gold juice and filtrate provided evidence regarding effective bacterial components in gold juice, which may aid in clinical decisions concerning fecal microbiota transplantation.

摘要

目的

鉴定金汁中潜在有效的细菌成分，金汁是一种用于粪便微生物群移植的中医疗法。

方法

从5名健康儿童（2名男孩和3名女孩；平均年龄7.52±2.31岁）采集粪便样本。这些儿童在之前3个月内无抗生素使用史或肠道微生态制剂使用史。于11月中下旬按照中医疗法从儿童中采集新鲜粪便样本制备金汁，并在7天内使用。最后，采用16S rDNA序列分析鉴定金汁中潜在有效的细菌成分。使用QIIME软件比较金汁、稀释剂、滤液和黄土样本中的微生物种类。

结果

按照“古法”处理后，金汁的微生物区系发生了显著变化。金汁样本和滤液样本中的微生物成分存在显著差异。我们研究中分析的金汁由通过降解物质合成碳水化合物和氨基酸的微生物组成，而滤液中含有益生菌菌群，如双歧杆菌属、嗜酸乳杆菌属和粪肠球菌。

结论

这项对金汁和滤液中微生物成分的研究为金汁中有效的细菌成分提供了证据，这可能有助于粪便微生物群移植的临床决策。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/a9387c599e59/10.1177_0300060520931288-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/9af0d4a50639/10.1177_0300060520931288-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/3069ec47f6e5/10.1177_0300060520931288-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/15f6e7186676/10.1177_0300060520931288-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/80eeececc839/10.1177_0300060520931288-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/6de4bea77f8c/10.1177_0300060520931288-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/e9eed5d155e2/10.1177_0300060520931288-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/e4f288b4c361/10.1177_0300060520931288-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/a9387c599e59/10.1177_0300060520931288-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/9af0d4a50639/10.1177_0300060520931288-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/3069ec47f6e5/10.1177_0300060520931288-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/15f6e7186676/10.1177_0300060520931288-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/80eeececc839/10.1177_0300060520931288-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/6de4bea77f8c/10.1177_0300060520931288-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/e9eed5d155e2/10.1177_0300060520931288-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/e4f288b4c361/10.1177_0300060520931288-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd1c/7545780/a9387c599e59/10.1177_0300060520931288-fig8.jpg

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