Xu Leifeng, Yang Panpan, Yuan Suxia, Feng Yayan, Xu Hua, Cao Yuwei, Ming Jun
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2016 Nov 20;17(11):1881. doi: 10.3390/ijms17111881.
Lily tepals have a short lifespan. Once the tepals senesce, the ornamental value of the flower is lost. Some cultivars have attractive purple ovaries and fruits which greatly enhance the ornamental value of Asiatic hybrid lilies. However, little is known about the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries. To investigate the transcriptional network that governs purple ovary coloration in Asiatic hybrid lilies, we obtained transcriptome data from green ovaries (S1) and purple ovaries (S2) of Asiatic "Tiny Padhye". Comparative transcriptome analysis revealed 4228 differentially expressed genes. Differential expression analysis revealed that ten unigenes including four genes, one gene, one gene, one gene, one gene, one gene, and one gene were significantly up-regulated in purple ovaries. One gene, , was identified as a key transcription factor determining the distribution of anthocyanins in Asiatic hybrid lily ovaries. Further qPCR results showed unigenes related to anthocyanin biosynthesis were highly expressed in purple ovaries of three purple-ovaried Asiatic hybrid lilies at stages 2 and 3, while they showed an extremely low level of expression in ovaries of three green-ovaried Asiatic hybrid lilies during all developmental stages. In addition, shading treatment significantly decreased pigment accumulation by suppressing the expression of several unigenes related to anthocyanin biosynthesis in ovaries of Asiatic "Tiny Padhye". Lastly, a total of 15,048 Simple Sequence Repeats (SSRs) were identified in 13,710 sequences, and primer pairs for SSRs were designed. The results could further our understanding of the molecular mechanisms of anthocyanin biosynthesis in Asiatic hybrid lily ovaries.
百合的花被片寿命较短。一旦花被片衰老，花朵的观赏价值就会丧失。一些品种具有吸引人的紫色子房和果实，这大大提高了亚洲杂种百合的观赏价值。然而，关于亚洲杂种百合子房花青素生物合成的分子机制知之甚少。为了研究调控亚洲杂种百合紫色子房着色的转录网络，我们从亚洲“小帕迪”的绿色子房（S1）和紫色子房（S2）中获得了转录组数据。比较转录组分析揭示了4228个差异表达基因。差异表达分析显示，包括四个 基因、一个 基因、一个 基因、一个 基因、一个 基因、一个 基因和一个 基因在内的十个单基因在紫色子房中显著上调。一个 基因， ，被鉴定为决定亚洲杂种百合子房花青素分布的关键转录因子。进一步的qPCR结果表明，与花青素生物合成相关的单基因在三个紫色子房的亚洲杂种百合的第2和第3阶段的紫色子房中高表达，而在三个绿色子房的亚洲杂种百合的子房在所有发育阶段均表现出极低的表达水平。此外，遮光处理通过抑制亚洲“小帕迪”子房中转录组分析中几个与花青素生物合成相关的单基因的表达，显著降低了色素积累。最后，在13710个序列中总共鉴定出15048个简单序列重复（SSR）并设计了SSR的引物对。这些结果可以加深我们对亚洲杂种百合子房花青素生物合成分子机制的理解。
