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雅克花粉蛋白过敏原对白化小鼠的影响。

Effect of Jacq. pollen protein allergen in albino mice.

作者信息

Ezikanyi Dimphna Nneka, Oselebe Happiness

机构信息

Palynology Unit, Department of Applied Biology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria.

Department of Crop Science, Faculty of Agriculture, Ebonyi State University, Abakaliki, Nigeria.

出版信息

Postepy Dermatol Alergol. 2020 Aug;37(4):566-571. doi: 10.5114/ada.2020.98286. Epub 2020 Sep 2.

DOI:10.5114/ada.2020.98286
PMID:32994780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7507149/
Abstract

INTRODUCTION

Pollen trigger allergenic reactions in hypersensitive individuals due to the presence of protein in their sporoderm.

AIM

Pollen protein produced by was subjected to allergenicity test in mice in order to determine its allergenic potential.

MATERIAL AND METHODS

Pollen protein was extracted using 0.02 M phosphate buffered saline and used to inoculate mice by two subcutaneous and one intranasal injections weekly for 4 weeks. Blood samples were obtained by retro-orbital bleedings, sera obtained were used in detecting immunoglobulin E (IgE) by immunoperoxidase assay. Values of immune cell and IgE elicited in mice were analysed using SPSS statistical package version 20.

RESULTS

yielded 208 µg/ml pollen protein allergen. Inoculation of pollen protein in mice produced dermatophytic allergic reactions which physically presented as swelling, rashes and hair loss. Pollen protein skewed basophil production and infiltration of lymphocytes by 1-62% and 58-99% respectively in relation to controls. Histopathology analysis showed inflammation within the lung parenchyma.

CONCLUSIONS

The present study is the first study to evaluate the allergenic potential of in mice. The findings revealed that which is profoundly planted in pubic areas for aesthetic purposes possess allergenic effect.

摘要

引言

由于花粉外壁中存在蛋白质,花粉会引发过敏个体的过敏反应。

目的

对由[未提及具体来源]产生的花粉蛋白进行小鼠过敏试验,以确定其过敏潜力。

材料与方法

用0.02M磷酸盐缓冲盐水提取花粉蛋白,每周通过两次皮下注射和一次鼻内注射对小鼠进行接种,持续4周。通过眶后放血获取血样,所得血清用于免疫过氧化物酶法检测免疫球蛋白E(IgE)。使用SPSS统计软件20版分析小鼠体内引发的免疫细胞和IgE值。

结果

[未提及具体来源]产生208μg/ml花粉蛋白过敏原。给小鼠接种花粉蛋白会产生皮肤过敏反应,表现为肿胀、皮疹和脱发。与对照组相比,花粉蛋白使嗜碱性粒细胞生成和淋巴细胞浸润分别偏移1 - 62%和58 - 99%。组织病理学分析显示肺实质内有炎症。

结论

本研究是首次评估[未提及具体来源]在小鼠体内过敏潜力的研究。研究结果表明,出于美观目的大量种植于公共区域的[未提及具体来源]具有过敏作用。

需注意,原文中部分关键信息缺失(如产生花粉蛋白的主体等),翻译后的内容在逻辑完整性上会受一定影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/ef4a9d857e4c/PDIA-37-98286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/7de37da10608/PDIA-37-98286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/1951c3c213ab/PDIA-37-98286-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/5074dea992ed/PDIA-37-98286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/ef4a9d857e4c/PDIA-37-98286-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/7de37da10608/PDIA-37-98286-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/1951c3c213ab/PDIA-37-98286-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/5074dea992ed/PDIA-37-98286-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/792b/7507149/ef4a9d857e4c/PDIA-37-98286-g004.jpg

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