Golestani Nadia, Khoshkhoo Payam Haghighi, Hosseini Hossein, Azad Gita Akbari
Department of Clinical Sciences, School of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
Iran J Microbiol. 2020 Aug;12(4):331-337. doi: 10.18502/ijm.v12i4.3937.
, an obligate intracellular, Gram-negative zoonotic pathogen, has eight serovars and nine genotypes isolated from avian species with higher frequency in parrots and pigeons. The aim of this study was to characterize spp. using nested PCR and sequencing.
A total of 270 pharyngeal swab samples collected randomly from asymptomatic pigeons of 30 pigeon aviaries in Tehran province. DNA was extracted with specific kit and amplified by specific primers in the first PCR and outer membrane protein A gene in the second PCR. Positive samples were sequenced and phylogenetic tree analyzed based on the gene.
Records showed that 16 of 30 (53%) pigeon aviaries were positive for spp. Phylogenetic tree analysis revealed that 15 of 16 (93.7%) positive samples, belonged to genotype B whereas the other sample belonged to detected in 50% of pigeon aviaries that is high rate in Tehran province.
As is a zoonosis and life threaten pathogen for human being, these results indicate the significance of it detection in asymptomatic pigeons. Also, this is the first report of presence in Iranian pigeons which its zoonotic potential is still unknown.
[病原体名称]是一种专性细胞内寄生的革兰氏阴性人畜共患病原体，有八个血清型和九个基因型，在鹦鹉和鸽子等禽类中分离频率较高。本研究的目的是利用巢式PCR和测序对[病原体名称]进行特征分析。
从德黑兰省30个鸽舍的无症状鸽子中随机采集了270份咽拭子样本。用特定试剂盒提取DNA，在第一次PCR中用特定引物扩增，在第二次PCR中扩增外膜蛋白A基因。对阳性样本进行测序，并基于该基因分析系统发育树。
记录显示，30个鸽舍中有16个（53%）检测到[病原体名称]呈阳性。系统发育树分析显示，16个阳性样本中有15个（93.7%）属于基因型B，而另一个样本属于在50%的鸽舍中检测到的[病原体名称]，这在德黑兰省的发生率较高。
由于[病原体名称]是一种人畜共患病，对人类生命构成威胁，这些结果表明在无症状鸽子中检测它的重要性。此外，这是伊朗鸽子中存在[病原体名称]的首次报告，其人畜共患病潜力仍未知。
