Wang Jing-Xian, Ming Xin, Tao Yan-Bin, Xu Zeng-Fu
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Innovation Academy for Seed Design, Chinese Academy of Sciences, Menglun, Mengla, Yunnan, China.
PeerJ. 2020 Sep 17;8:e9827. doi: 10.7717/peerj.9827. eCollection 2020.
L., a perennial oilseed plant, is considered as a promising feedstock for biodiesel production. Genetic modification of flowering characteristics is critical for breeding. However, analysis of floral-specific promoters in is limited.
In this study, we isolated the ortholog of () gene from flower cDNA library and detected the expression pattern of gene by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). We isolated a 1.8-kb fragment from the 5' region of the gene and evaluated its spatiotemporal expression pattern in using the - () reporter gene and () gene, respectively.
was identified as a flower-specific gene in . As expected, promoter was only active in transgenic flowers with the strongest activity in stamens. Moreover, transgenic showed a phenotype of large flowers without any alterations in other organs. Furthermore, deletion of the region from -1,717 to -876 bp resulted in the disappearance of promoter activity in stamens but an increase in promoter activity in young leaves, sepals, and petals. Deletion analysis suggests that the -1,717- to -876-bp promoter fragment contains regulatory elements that confer promoter activity in stamens and inhibit activity in young leaves, sepals, and petals.
L. 是一种多年生油料植物，被认为是生物柴油生产的一种有前景的原料。开花特性的基因改造对育种至关重要。然而，对L. 中花特异性启动子的分析有限。
在本研究中，我们从L. 花cDNA文库中分离出FLC基因的直系同源基因，并通过定量逆转录-聚合酶链反应（qRT-PCR）检测该基因的表达模式。我们从FLC基因的5'区域分离出一个1.8 kb的片段，并分别使用GUS（β-葡萄糖苷酶）报告基因和GFP（绿色荧光蛋白）基因评估其在L. 中的时空表达模式。
FLC被鉴定为L. 中的花特异性基因。正如预期的那样，FLC启动子仅在转基因L. 花中具有活性，在雄蕊中活性最强。此外，FLC转基因L. 表现出大花的表型，而其他器官没有任何改变。此外，缺失-1717至-876 bp区域导致雄蕊中启动子活性消失，但幼叶、萼片和花瓣中的启动子活性增加。缺失分析表明，-1717至-876 bp的启动子片段包含赋予雄蕊启动子活性并抑制幼叶、萼片和花瓣中活性的调控元件。
