Schraer H, Gay C V
Calcif Tissue Res. 1977 Jun 28;23(2):185-8. doi: 10.1007/BF02012785.
Matrix vesicles were observed in femurs of 8-day-old chick embryos prepared by ultracryotomy. Some of the sections were subjected to ultramicroincineration. The unfixed tissues never came into contact with solutions, and thereby artifacts due to dissolution, redistribution, or rearrangement of the mineral constituents were avoided. In the osteoid, electron dense objects with the size and appearance of matrix vesicles were seen, although limiting membranes were not visible. After ultramicroincineration the vesicles were observed to contain small crystals and a less dense amorphous mineral material which may te the precursor of bone mineral. In addition, a ring of ash enclosed the crystalline and amorphous mineral and appeared to occupy the position of the vesicle membrane as seen in conventionally prepared material.
通过超薄切片术在8日龄鸡胚的股骨中观察到基质小泡。部分切片进行了超微灰化。未固定的组织从未与溶液接触，从而避免了由于矿物质成分溶解、重新分布或重排而产生的假象。在类骨质中，可见到具有基质小泡大小和外观的电子致密物体，尽管看不到界限膜。超微灰化后，观察到小泡含有小晶体和密度较低的无定形矿物质，这可能是骨矿物质的前体。此外，在传统制备的材料中可见，一圈灰包围着晶体和无定形矿物质，似乎占据了小泡膜的位置。
