Institute of Biology, Department of Invertebrate Systematics and Ecology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631, Wrocław, Poland.
Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia, 199034.
Exp Appl Acarol. 2020 Nov;82(3):359-378. doi: 10.1007/s10493-020-00553-8. Epub 2020 Sep 29.
The morphology and formation of stylostomes (feeding tubes) in hosts' body during the parasitic phase of Allothrombium fuliginosum (Hermann) larvae were studied for the first time with light microscopy (LM) and transmission electron microscopy (TEM). The stylostomes were observed in three aphids species-Acyrthosiphon pisum (Harris), Elatobium abietinum (Walker), and Macrosiphum rosae (L.)-parasitized by mites under laboratory conditions. They consisted of 2-6 main branches, preliminarily unbranched, then producing secondary and sometimes also tertiary branches as finally formed structures. Their walls were uniformly electron-dense, without any longitudinal and transverse stratifications and showed rather irregular outlines. Distally, the stylostome branches revealed transparent pores and cavities in their walls, connecting the stylostome canal with surrounding haemocoelic space. The total length of stylostomes at the end of the parasitic phase was on average 16× greater than that recorded in the youngest stylostomes. No differences in the overall shape of feeding tubes between host species were stated. The stylostomes formed in different host species did not differ significantly, except their total length, which attained the highest value in tissues of Ac. pisum.
首次利用光学显微镜（LM）和透射电子显微镜（TEM）研究了 Allothrombium fuliginosum（Hermann）幼虫寄生阶段宿主体内吸管（进食管）的形态和形成过程。在实验室条件下，观察到三种蚜虫物种——豌豆蚜（Harris）、白松叶蜂（Walker）和桃蚜（L.）——被螨虫寄生时的吸管。它们由 2-6 个主要分支组成，最初是不分枝的，然后产生二次甚至三次分支，最终形成结构。它们的壁均匀电子致密，没有任何纵向和横向分层，并且呈现出相当不规则的轮廓。在远端，吸管分支在其壁上显示出透明的孔和腔，将吸管通道与周围血腔空间连接起来。寄生阶段结束时，吸管的总长度平均比最年轻的吸管长 16 倍。在宿主物种之间，没有观察到进食管的整体形状有任何差异。在不同宿主物种中形成的吸管没有明显差异，除了它们的总长度，在豌豆蚜的组织中达到最高值。
