Department of Neurosurgery, Fudan University Shanghai Cancer Center, Shanghai, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
J Cell Mol Med. 2020 Nov;24(22):13010-13019. doi: 10.1111/jcmm.15899. Epub 2020 Sep 29.
Long noncoding RNAs have key roles in glioma progression. However, the function and mechanisms of action of the long noncoding RNA, LINC00346, in glioma remain unclear. In our study, we observed that LINC00346 levels were increased in glioma tissue samples, and according to Gene Expression Profiling Interactive Analysis, its levels were related to disease-free survival and overall survival rates, suggesting that a high level of LINC00346 expression corresponds to a poor prognosis. We next confirmed the high levels of LINC00346 expression in glioma tissues and cell lines and showed that LINC00346 knockdown suppressed glioma cell proliferation, migration and invasion; promoted apoptosis; and delayed tumour growth. Moreover, the oncogenic function of LINC00346 may be explained, in part, by the down-regulation of miR-340-5p and the de-repression of ROCK1. We showed that LINC00346 may function as a competing endogenous RNA of miR-340-5p, thereby de-repressing ROCK1. This study revealed a new regulatory network in glioma and identified potential therapeutic targets for this cancer.
长非编码 RNA 在神经胶质瘤的进展中发挥着关键作用。然而，长非编码 RNA LINC00346 在神经胶质瘤中的功能和作用机制仍不清楚。在我们的研究中，我们观察到 LINC00346 的水平在神经胶质瘤组织样本中增加，并且根据基因表达谱分析交互分析，其水平与无病生存率和总生存率相关，表明高水平的 LINC00346 表达与不良预后相关。我们接下来证实了 LINC00346 在神经胶质瘤组织和细胞系中的高表达水平，并表明 LINC00346 敲低抑制了神经胶质瘤细胞的增殖、迁移和侵袭；促进了细胞凋亡；并延缓了肿瘤生长。此外，LINC00346 的致癌功能部分可以通过下调 miR-340-5p 和抑制 ROCK1 来解释。我们表明 LINC00346 可能作为 miR-340-5p 的竞争性内源 RNA 发挥作用，从而解除对 ROCK1 的抑制。本研究揭示了神经胶质瘤中的一个新的调控网络，并为这种癌症确定了潜在的治疗靶点。
