Department of Biochemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iran J Immunol. 2020 Sep;17(3):215-225. doi: 10.22034/iji.2020.85513.1715.
According to genome wide association studies, SLC30A8 is among the loci containing SNPs associated with type 2 diabetes (T2D) risk. This gene encodes an islet zinc transporter (ZnT8).
To provide new information on the association of the SNP rs11558471 in SLC30A8 gene with IL-17 levels and insulin resistance in an Iranian population with T2D.
A total of 133 patients with T2D and 128 control subjects were included in this study. Insulin and IL-17 concentrations were determined using ELISA. Insulin and fasting blood glucose levels were employed to determine homeostasis model assessment for insulin resistance (HOMA-IR). PCR-based restriction fragment length polymorphism was performed to determine rs11558471 polymorphism.
The risk allele frequency of rs11558471 in studied population was among the highest frequencies in different populations. In T2D patients, compared with the GG genotypes, IL-17 concentrations were significantly higher in the GA+AA group (p=0.042). According to the genotypes of this SNP, IL-17 concentrations, fasting blood glucose and HOMA-IR increased with the following order: GG<GA<AA. A multiple regression revealed that following an adjustment for age and gender, rs11558471 as an independent variable was significantly associated with IL-17 (p=0.039), fasting blood glucose (p=0.003) and HOMA-IR (p=0.042) as the dependent variables.
The present study demonstrated for the first time, the genetic association of rs11558471 with IL-17 and glycemic traits in Iranian patients with T2D. The association of rs11558471 with glycemic traits showed that it might be useful for the identification of individuals who are at high risk for the development of T2D.
根据全基因组关联研究，SLC30A8 是与 2 型糖尿病（T2D）风险相关的 SNP 所在的基因座之一。该基因编码胰岛锌转运体（ZnT8）。
为了提供伊朗 T2D 人群中 SLC30A8 基因中 SNP rs11558471 与 IL-17 水平和胰岛素抵抗之间关联的新信息。
本研究共纳入 133 例 T2D 患者和 128 例对照者。采用 ELISA 法测定胰岛素和 IL-17 浓度。采用稳态模型评估胰岛素抵抗（HOMA-IR）计算胰岛素和空腹血糖水平。采用 PCR 基础限制性片段长度多态性检测 rs11558471 多态性。
在研究人群中，rs11558471 的风险等位基因频率属于不同人群中的最高频率之一。与 GG 基因型相比，T2D 患者中 GA+AA 组的 IL-17 浓度显著升高（p=0.042）。根据该 SNP 的基因型，IL-17 浓度、空腹血糖和 HOMA-IR 呈 GG<GA<AA 的顺序升高。多元回归分析显示，在调整年龄和性别后，rs11558471 作为一个独立变量与 IL-17（p=0.039）、空腹血糖（p=0.003）和 HOMA-IR（p=0.042）呈显著相关。
本研究首次证明了 rs11558471 与伊朗 T2D 患者的 IL-17 和血糖特征之间的遗传关联。rs11558471 与血糖特征的关联表明，它可能有助于识别发生 T2D 风险较高的个体。
