Animal Facility of the Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
Department of Gene Therapy and Molecular Immunology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
PLoS One. 2020 Sep 30;15(9):e0239231. doi: 10.1371/journal.pone.0239231. eCollection 2020.
It is controversially discussed whether immune-deficient mice experience severity in the absence of infection. Because a comprehensive analysis of the well-being of immune-deficient mice under specific pathogen free conditions is missing, we used a multi-parametric test analyzing, corticosterone, weight, nest building and facial expression over a period of 9 month to determine the well-being of two immune-deficient mouse lines (recombination activating gene 2- and interferon gamma receptor-deficient mice). We do not find evidence for severity when comparing immune-deficient mice to their heterozygous immune-competent littermates. Our data challenge the assumption that immune-deficiency per se regardless of housing conditions causes severity. Based on our study we propose to use objective non-invasive parameters determined by laboratory animal science for decisions concerning severity of immune-deficient mice.
免疫缺陷小鼠在没有感染的情况下是否会经历严重程度，这是一个有争议的问题。由于缺乏对特定病原体条件下免疫缺陷小鼠整体健康状况的综合分析，我们使用多参数测试分析了糖皮质激素、体重、筑巢和面部表情，以确定两种免疫缺陷小鼠系（重组激活基因 2 和干扰素γ受体缺陷小鼠）的健康状况。与杂合免疫功能正常的同窝小鼠相比，我们没有发现免疫缺陷小鼠严重程度的证据。我们的数据挑战了这样一种假设，即无论饲养条件如何，免疫缺陷本身都会导致严重程度。基于我们的研究，我们建议使用实验室动物科学确定的客观非侵入性参数来决定免疫缺陷小鼠的严重程度。
