Department of Entomology, Arak Branch, Islamic Azad University, Arak, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Mitochondrial DNA A DNA Mapp Seq Anal. 2020 Dec;31(8):327-334. doi: 10.1080/24701394.2020.1815718. Epub 2020 Oct 1.
Information on the genetic structure of rice striped stem borer, (Walker), is essential for an effective pest management program and can corporate with control program and prompt the modification of current control methods. In this study, the genetic structure of COI gene has been investigated for 18 populations of this pest in north of Iran. specimens were collected from rice fields on rice and weeds in Guilan and Mazanderan provinces, at Caspian Sea coast at both seasonal activity (spring) and overwintering stage from May through October 2018 and identified using morphological characters. Genetic structure of 18 populations of the species was evaluated using polymerase chain reaction (PCR)-direct-sequencing of a fragment (869 bp) of mtDNA-COI gene. Results revealed that almost all specimens from different generations, hosts, and locations belong to a single haplotype very close to a South Korean haplotype. To the best our knowledge, it is the first comprehensive molecular typing of striped stem borer in both Northern provinces of the country and suggests a high gene flow among different populations of this pest in the region.
有关水稻条螟（Walker）遗传结构的信息对于有效的害虫管理计划至关重要，它可以与控制计划相结合，并促使当前控制方法的修改。在这项研究中，对伊朗北部 18 个种群的 COI 基因的遗传结构进行了调查。 2018 年 5 月至 10 月，从吉兰省和马赞达兰省的稻田以及里海沿岸的杂草上采集了标本，在春季和越冬期利用形态学特征对其进行了鉴定。使用聚合酶链反应（PCR）-直接测序法对 mtDNA-COI 基因的一个片段（869 bp）对 18 个种群的遗传结构进行了评估。结果表明，来自不同世代、宿主和地点的几乎所有标本都属于与韩国单倍型非常接近的单一单倍型。据我们所知，这是对该国北部两个省份的条螟进行的首次全面分子分型，表明该地区不同种群之间存在很高的基因流。
