Huang Min, Zhao Qiyu, Ye Zhengdu, Xu Danxia, Tang Shi, Jiang Tian'an
Department of Ultrasound, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province 310003, China.
Anal Methods. 2020 Oct 7;12(37):4556-4561. doi: 10.1039/d0ay01135e. Epub 2020 Sep 2.
Hepatocellular carcinoma (HCC) is a common malignancy worldwide with poor prognosis. The early identification and precise resection of HCC are essential for improving the prognosis and overall survival of patients. In clinical practice, fluorescence imaging is a powerful technology to identify and remove HCC lesions, but accurate and reliable detection of HCC continues to remain a challenge due to non-specificity and false-positive uptake of probes. To circumvent these problems, it is crucial to design a specific probe for the accurate detection of HCC. Herein, we reported the design and synthesis of an NIR fluorescent probe by conjugating IRDye800CW with melatonin, which plays a significant role in the HCC development. The in vivo imaging revealed that IRDye800-MT was uptake specifically by the HCC tumor with a high tumor-to-background ratio. These results demonstrated that IRDye800-MT might hold clinical potentials for future diagnosis of HCC patients.
肝细胞癌（HCC）是一种全球常见的恶性肿瘤，预后较差。HCC的早期识别和精确切除对于改善患者的预后和总体生存率至关重要。在临床实践中，荧光成像技术是识别和切除HCC病灶的有力手段，但由于探针的非特异性和假阳性摄取，HCC的准确可靠检测仍然是一个挑战。为了规避这些问题，设计一种用于准确检测HCC的特异性探针至关重要。在此，我们报道了一种通过将IRDye800CW与褪黑素偶联而设计合成的近红外荧光探针，褪黑素在HCC发展中起重要作用。体内成像显示，IRDye800-MT被HCC肿瘤特异性摄取，肿瘤与背景比值高。这些结果表明，IRDye800-MT在未来HCC患者诊断中可能具有临床潜力。
