Singh Amandeep, Singh Opinder
Department of Veterinary Anatomy, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, India.
Department of Veterinary Anatomy, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India.
Anat Histol Embryol. 2021 Mar;50(2):250-259. doi: 10.1111/ahe.12622. Epub 2020 Oct 1.
The present study was aimed at elucidating the histogenesis of parotid gland of buffalo. The study was carried out on buffalo foetuses (n = 36), during different stages of prenatal life. The foetuses were categorised into three groups based on their curved crown rump length (CVRL). The primordial anlage of parotid salivary gland was evident at 40th day of development whereas the primary ducts, in the form of cords, were first observed at 81st day of prenatal life. The capsule formation as well as the lobulation of the gland was initiated at 127th day. At 141st day, the duct system of gland was completed. The terminal tubules attained the structure of acini at 167th day. The myoepithelial cells first appeared as flattened basal cells initially around the developing acinar cells at 167th day. The typical compound tubulo-acinar nature of the gland was first observed at 185th day. Purely serous acinar cells were seen from 185th day onwards. The micrometrical studies revealed that the mean diameter of acinar cells, intercalated ducts, striated ducts and large ducts increased with the advancement of age. The serous acinar cells were devoid of acidic as well as neutral mucopolysaccharides in prenatal age groups; however, large ducts with goblet cells exhibited positive reaction. Combined PAS-AB method revealed mixed reaction in acinar cells as well as in large ducts. Fine lipid droplets were observed in intralobular as well as interlobular connective tissue; however, phospholipids were observed in the cell membrane of secretory cells and ducts.
本研究旨在阐明水牛腮腺的组织发生过程。该研究以水牛胎儿（n = 36）为对象，在产前生活的不同阶段进行。根据胎儿的曲冠臀长（CVRL）将其分为三组。腮腺唾液腺的原基在发育第40天可见，而呈索状的初级导管在产前生活第81天首次观察到。腺体的被膜形成以及小叶化在第127天开始。在第141天，腺体的导管系统发育完成。终末小管在第167天获得腺泡结构。肌上皮细胞在第167天首次以扁平的基底细胞形式出现在发育中的腺泡细胞周围。腺体典型的复管泡状性质在第185天首次观察到。从第185天起可见纯浆液性腺泡细胞。显微测量研究表明，腺泡细胞、闰管、纹状管和主导管的平均直径随着年龄的增长而增加。在产前年龄组中，浆液性腺泡细胞不含酸性和中性粘多糖；然而，含有杯状细胞的主导管呈阳性反应。联合PAS-AB法显示腺泡细胞和主导管呈混合反应。在小叶内和小叶间结缔组织中观察到细小脂滴；然而，在分泌细胞和导管的细胞膜中观察到磷脂。
