School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland.
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia.
Sensors (Basel). 2020 Sep 29;20(19):5594. doi: 10.3390/s20195594.
This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.
这篇综述重点介绍了用于检测胰蛋白酶和纤溶酶等牛奶蛋白酶的表面和体积敏感声学法的应用。虽然胰蛋白酶是人乳中的重要蛋白质，但纤溶酶是一种在牛、绵羊和山羊乳质量中起重要作用的蛋白酶。纤溶酶活性的增加会导致β-酪蛋白的广泛裂解，从而影响牛奶的胶凝和口感。本文介绍了表面敏感声学法的基本原理以及高分辨率超声光谱（HR-US）。讨论了实时应用声传感器检测蛋白酶的最新实例。展示了 HR-US 方法在研究酶反应动力学中的应用。比较了声学生物传感器和 HR-US 方法检测蛋白酶的灵敏度。
