Warnock M L, Sniezek M, Shellito J
Am J Pathol. 1987 Jul;128(1):171-80.
To determine whether the cytochemical localization of peroxidase activity could be used as a marker of monocyte influx into the lung during an inflammatory response, the authors studied the peroxidase phenotypes of lavaged alveolar macrophages from rats with bacille Calmette-Guérin (BCG)-induced pulmonary inflammation. Rats were immunized subcutaneously and 2 weeks later intravenously with BCG. During the early phase of pulmonary inflammation, an increase was observed in the numbers of alveolar macrophages with no peroxidase activity in the endoplasmic reticulum. These cells appeared to reflect monocyte influx into the injured lung. The later stages of inflammation were characterized by increased numbers of alveolar macrophages with peroxidase-positive endoplasmic reticulum, probably due to activation of enzymatic activity in situ. During the early phase, peroxidase activity was also observed within macrophage cytoplasmic inclusions, probably representing both primary monocyte lysosomes and internalized myeloperoxidase from inflammatory neutrophils. Serial observations indicated that the peroxidase-positive cytoplasmic inclusions became negative with time. It is concluded that inflammation-induced modulation of peroxidase activity in the endoplasmic reticulum and in cytoplasmic inclusions makes the alveolar macrophage peroxidase phenotype no more than a rough marker of monocyte influx into the inflamed lung.
为了确定过氧化物酶活性的细胞化学定位是否可作为炎症反应期间单核细胞流入肺部的标志物，作者研究了卡介苗（BCG）诱导的肺部炎症大鼠灌洗肺泡巨噬细胞的过氧化物酶表型。大鼠皮下免疫，2周后静脉注射BCG。在肺部炎症的早期阶段，观察到内质网中无过氧化物酶活性的肺泡巨噬细胞数量增加。这些细胞似乎反映了单核细胞流入受损肺部。炎症后期的特征是内质网过氧化物酶阳性的肺泡巨噬细胞数量增加，这可能是由于原位酶活性的激活。在早期阶段，在巨噬细胞胞质内含物中也观察到过氧化物酶活性，这些内含物可能代表原发性单核细胞溶酶体和来自炎症中性粒细胞的内化髓过氧化物酶。连续观察表明，过氧化物酶阳性的胞质内含物随时间变为阴性。得出的结论是，炎症诱导的内质网和胞质内含物中过氧化物酶活性的调节使得肺泡巨噬细胞过氧化物酶表型不过是单核细胞流入炎症肺部的一个粗略标志物。
