Lee K C, Wong M, McIntyre D
J Immunol. 1981 Jun;126(6):2474-9.
The subpopulations of murine macrophages (M phi) that can be activated by endotoxin (LPS) and lymphokines to produce lymphocyte-activating factor, or interleukin 1 (IL-1), and to express cytostatic and cytolytic activities against tumor cells were characterized in 3 cell populations with immature and mature M phi in various proportions, namely, normal peritoneal cells, glycogen-induced peritoneal cells, and cultured bone marrow M phi. There is disagreement in the literature as to whether tumor cytotoxicity can be elicited from all M phi or from a subset of small, newly formed peroxidase-positive (p+) monocytes. Using the technique of cell size fractionation, which separates p+ and p- M phi to various extents, we have found that the magnitude of te response to LPS and lymphokines increases with cell size (and hence maturity or the degree of prior stimulation) and is not exclusive to p+ M phi. Our results are consistent with the concept that all M phi, irrespective of developmental stage, can exist in a continuum of activated states, depending on the nature of the activation signals.
能被内毒素（LPS）和淋巴因子激活以产生淋巴细胞激活因子即白细胞介素1（IL-1），并对肿瘤细胞表现出抑制细胞生长和细胞溶解活性的小鼠巨噬细胞（M phi）亚群，在具有不同比例未成熟和成熟M phi的3种细胞群体中得到了表征，这3种细胞群体分别是正常腹腔细胞、糖原诱导腹腔细胞和培养的骨髓M phi。关于肿瘤细胞毒性是能从所有M phi中诱导产生，还是仅从小的、新形成的过氧化物酶阳性（p+）单核细胞亚群中诱导产生，文献中存在分歧。使用能在不同程度上分离p+和p- M phi的细胞大小分级技术，我们发现对LPS和淋巴因子的反应强度随细胞大小增加（因此也随成熟度或先前刺激程度增加），且并非p+ M phi所特有。我们的结果与以下概念一致，即所有M phi，无论发育阶段如何，都可以根据激活信号的性质处于连续的激活状态。
