Li Jijun, Guo Yi, Zhao Lin, Sun Kexing, Xi Guangjun, Li Zai-Wang
Department of Integrative Medicine on Pediatrics, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
Department of Neurology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiiated Hospital of Southern University of Science and Technology, Shenzhen 518020, China.
Evid Based Complement Alternat Med. 2020 Sep 12;2020:2980705. doi: 10.1155/2020/2980705. eCollection 2020.
This study aimed to evaluate the possible mechanism of Ningdong granule (NDG) for the treatment of Tourette syndrome (TS). The rats with stereotyped behavior were established by microinjection with TS patients' sera; then, the model rats were divided into NDG and haloperidol (Hal) group, and the nonmedication model rats were regarded as treatment control (TS group). The stereotyped behavior of the rats was recorded, the level of dopamine (DA) in striatum, and the content of homovanillic acid (HVA) in sera were tested, and dopamine transporter (DAT) expression was measured in the study. The experimental results showed that NDG effectively inhibited the stereotyped behavior ( < 0.01), decreased the levels of DA in the striatum ( < 0.05), increased the content of sera HVA ( < 0.01), and enhanced the protein and mRNA expression of DAT in the striatum ( < 0.01). Additionally, the results also revealed Hal could improve the stereotyped behavior as well but had no remarkable influence on DAT expression and DA metabolism. In conclusion, NDG attenuates stereotyped behavior, and its mechanism of action might be associated with the upregulation of DAT expression to regulate DA metabolism in the brain.
本研究旨在评估宁动颗粒（NDG）治疗抽动秽语综合征（TS）的可能机制。通过微量注射TS患者血清建立具有刻板行为的大鼠模型；然后，将模型大鼠分为NDG组和氟哌啶醇（Hal）组，将未用药的模型大鼠作为治疗对照（TS组）。记录大鼠的刻板行为，检测纹状体中多巴胺（DA）水平以及血清中高香草酸（HVA）含量，并测定多巴胺转运体（DAT）表达。实验结果表明，NDG可有效抑制刻板行为（<0.01），降低纹状体中DA水平（<0.05），增加血清HVA含量（<0.01），并增强纹状体中DAT的蛋白和mRNA表达（<0.01）。此外，结果还显示Hal也可改善刻板行为，但对DAT表达和DA代谢无显著影响。总之，NDG可减轻刻板行为，其作用机制可能与上调DAT表达以调节脑内DA代谢有关。
