Fujita Hidetoshi, Aratani Satoko, Mizoguchi Izuru, Yagishita Naoko, Nakajima Toshihiro
Department of Locomotor Science, Institute of Medical Science, Tokyo Medical University, Tokyo 160-8402, Japan.
Department of Future Medical Science, Institute of Medical Science, Tokyo Medical University, Tokyo 160-8402, Japan.
Exp Ther Med. 2020 Nov;20(5):121. doi: 10.3892/etm.2020.9249. Epub 2020 Sep 21.
Obesity is currently a major medical and societal issue. Synoviolin (SYVN1) is an E3 ubiquitin ligase involved in endoplasmic reticulum (ER) stress. Overexpression of has been found in genetically obese mice (/ and /), and treatment with a inhibitor suppresses weight gain in some mouse models (C57BL/6J and /). However, expression in humans has not yet been elucidated. In the present study, 35 human volunteers were analyzed, and the expression level of mRNA in peripheral blood mononuclear cells (PBMCs) was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. Expression of mRNA was significantly increased in PBMCs from volunteers with a BMI ≥25.0, compared with volunteers with a BMI <25.0. In addition, PCR array and RT-qPCR of ER stress-responsive genes revealed that the expression of activating transcription factor 6 (ATF6), which plays an important role in the transcriptional activation of , was increased in PBMCs from volunteers with a BMI ≥25.0. These results suggest that the - axis might be an important pathway in the progression of obesity.
肥胖是当前一个主要的医学和社会问题。滑膜素（SYVN1）是一种参与内质网（ER）应激的E3泛素连接酶。在遗传性肥胖小鼠（/和/）中发现其过表达，并且在一些小鼠模型（C57BL/6J和/）中用一种抑制剂治疗可抑制体重增加。然而，其在人类中的表达尚未阐明。在本研究中，分析了35名人类志愿者，并用逆转录定量聚合酶链反应（RT-qPCR）分析检测外周血单个核细胞（PBMCs）中滑膜素mRNA的表达水平。与BMI<25.0的志愿者相比，BMI≥25.0的志愿者的PBMCs中滑膜素mRNA的表达显著增加。此外，ER应激反应基因的PCR阵列和RT-qPCR显示，在转录激活滑膜素中起重要作用的激活转录因子6（ATF6）在BMI≥25.0的志愿者的PBMCs中的表达增加。这些结果表明滑膜素轴可能是肥胖进展中的一条重要途径。
