Thayer D W, Muller W S, Buchanan R L, Phillips J G
Appl Environ Microbiol. 1987 Jun;53(6):1311-5. doi: 10.1128/aem.53.6.1311-1315.1987.
The interactions of pH (5.0, 6.0, and 7.0), temperature (19, 28, and 37 degrees C), and atmosphere (aerobic versus anaerobic) with NaCl (0, 1, 2, 3, 4, and 5%) on the growth of Salmonella typhimurium ATCC 14028 in defined glucose-mineral salts culture medium were evaluated. Response surface methodology was used to develop equations describing the response of S. typhimurium to environmental changes. The response to an increasing concentration of NaCl at any temperature tested was nonlinear. The maximum growth was predicted to occur at an NaCl concentration of 0.5%, a temperature of 19 degrees C, and an initial pH of 7.0 under aerobic growth conditions. The relative amounts of aerobic growth at 19 degrees C, pH 7.0, and NaCl concentrations of 0, 0.5, 1, 2, 3, 4, and 5% were predicted to be 99.2, 100.0, 98.8, 90.2, 73.5, 48.6, and 15.6%, respectively. Anaerobic growth conditions repressed the amount of growth relative to that under aerobic conditions, and the effects of NaCl and pH were additive at low salt concentrations; however, at higher salt levels anaerobiosis provided protection against the effects of NaCl.
评估了pH值（5.0、6.0和7.0）、温度（19、28和37摄氏度）以及有氧与无氧环境与0%、1%、2%、3%、4%和5%氯化钠对鼠伤寒沙门氏菌ATCC 14028在限定的葡萄糖-矿物质盐培养基中生长的相互作用。采用响应面法建立描述鼠伤寒沙门氏菌对环境变化响应的方程。在任何测试温度下，随着氯化钠浓度的增加，其响应呈非线性。预计在有氧生长条件下，氯化钠浓度为0.5%、温度为19摄氏度、初始pH值为7.0时生长达到最大值。预计在19摄氏度、pH值为7.0且氯化钠浓度分别为0%、0.5%、1%、2%、3%、4%和5%时，有氧生长的相对量分别为99.2%、100.0%、98.8%、90.2%、73.5%、48.6%和15.6%。与有氧条件相比，无氧生长条件抑制了生长量，并且在低盐浓度下氯化钠和pH值的影响具有叠加性；然而，在较高盐水平下，无氧环境可提供对氯化钠影响的保护作用。
